Anti-TCPTP Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-TCPTP Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-TCPTP magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-TCPTP Magnetic Beads-IP Kit Product Description
The Anti-TCPTP magnetic Beads, conjugated with Anti-TCPTP antibody, are used for immuneprecipitation (IP) of TCPTP proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing TCPTP proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound TCPTP proteins are dissociated from the magnetic beads using an elution buffer. Anti-TCPTP Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Mouse PTPN2 / TC-PTP protein (Catalog#50800-M07B)
Species Reactivity
Mouse PTPN2 / TC-PTP
Source
Polyclonal Mouse Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Mouse PTPN2 / TC-PTP (rh PTPN2 / TC-PTP; Catalog#50800-M07B; Q06180-1; Ser2-Asn314). PTPN2 / TC-PTP specific IgG was purified by Mouse PTPN2 / TC-PTP affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-TCPTP Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-AI325124ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-PtptALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-TC-PTPALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
TCPTP Background Information
Tyrosine-protein phosphatase non-receptor type 2, also known as T-cell protein-tyrosine phosphatase, PTPN2 and PTPT, is a cytoplasm protein which belongs to the protein-tyrosine phosphatase family and Non-receptor class 1 subfamily. Members of the protein tyrosine phosphatase ( PTP ) family share a highly conserved catalytic motif, which is essential for the catalytic activity. TC-PTP / PTPN2 is a cytosolic tyrosine phosphatase that functions as a negative regulator of a variety of tyrosine kinases and other signaling proteins. The expression of TC-PTP / PTPN2 plays a role of tumor suppressor and may modulate response to treatment. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. Epidermal growth factor receptor and the adaptor protein Shc were reported to be substrates of this PTP, which suggested the roles in growth factor mediated cell signaling. TC-PTP / PTPN2 is an enzyme that is essential for the proper functioning of the immune system and that participates in the control of cell proliferation, and inflammation. TC-PTP / PTPN2 was identified as a negative regulator of NUP214-ABL1 kinase activity.
Full Name
protein tyrosine phosphatase, non-receptor type 2
References
Cool D., et al.,(1989), cDNA isolated from a human T-cell library encodes a member of the protein-tyrosine-phosphatase family. Proc. Natl. Acad. Sci. U.S.A. 86:5257-5261. Ota T., et al., (2004), Complete sequencing and characterization of 21,243 full-length human cDNAs.Nat. Genet. 36:40-45. Nusbaum C., et al.,(2005), DNA sequence and analysis of human chromosome 18.Nature 437:551-555.
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