Anti-CD146 / MCAM Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-CD146 / MCAM Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-CD146 / MCAM magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-CD146 / MCAM Magnetic Beads-IP Kit Product Description
The Anti-CD146 / MCAM magnetic Beads, conjugated with Anti-CD146 / MCAM antibody, are used for immuneprecipitation (IP) of CD146 / MCAM proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing CD146 / MCAM proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound CD146 / MCAM proteins are dissociated from the magnetic beads using an elution buffer. Anti-CD146 / MCAM Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Mouse CD146 / MCAM protein (Catalog#50794-M08H)
Species Reactivity
Mouse CD146 / MCAM
Source
Polyclonal Mouse Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Mouse CD146 / MCAM (rM CD146 / MCAM; Catalog#50794-M08H; Q8R2Y2-1; Met1-Val563). CD146 / MCAM specific IgG was purified by Mouse CD146 / MCAM affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-CD146 / MCAM Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-1-gicerinALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-AV025631ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-CD146ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-CD149ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-Muc18ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-s-endoALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-s-gicerinALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
CD146 / MCAM Background Information
The CD146 antigen, also known as melanoma cell adhesion molecule (MCAM) and MUC18, is an integral membrane glycoprotein belonging to the immunoglobulin superfamily. CD146 contains the characteristic immunoglobulin-like domains (V-V-C2-C2-C2), a transmembrane region and a short cytoplasmic tail. The CD146 expression is detected in endothelial cells in vascular tissue throughout the body, and plays a role in cell adhesion, as well as in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. As a Ca2+-independent cell adhesion molecule involved in heterophilic cell to cell interactions and a surface receptor, CD146 triggers tyrosine phosphorylation of FYN and PTK2 and subsequently induced signal transduction, proteolysis, or immune recognition. This protein is also expressed predominantly on metastatic lesions and advanced primary tumours, and thus has been suggested to play an important role in tumour progression and the development of metastasis in certain human carcinomas.
Full Name
melanoma cell adhesion molecule
References
Mills L, et al. (2002) Fully human antibodies to MCAM/MUC18 inhibit tumor growth and metastasis of human melanoma. Cancer Res. 62(17): 5106-14. Taira E, et al. (2004) Characterization of Gicerin/MUC18/CD146 in the rat nervous system. J Cell Physiol. 198(3): 377-87. Fritzsche FR, et al. (2008) CD146 protein in prostate cancer: revisited with two different antibodies. Pathology. 40(5): 457-64. Bidlingmaier S, et al. (2009) Identification of MCAM/CD146 as the target antigen of a human monoclonal antibody that recognizes both epithelioid and sarcomatoid types of mesothelioma. Cancer Res. 69(4): 1570-7. Boneberg EM, et al. (2009) Soluble CD146 is generated by ectodomain shedding of membrane CD146 in a calcium-induced, matrix metalloprotease-dependent process. Microvasc Res. 78(3): 325-31.
新浪微博
腾讯微博
QQ空间
客服
