Anti-CA8 Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-CA8 Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-CA8 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-CA8 Magnetic Beads-IP Kit Product Description
The Anti-CA8 magnetic Beads, conjugated with Anti-CA8 antibody, are used for immuneprecipitation (IP) of CA8 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing CA8 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound CA8 proteins are dissociated from the magnetic beads using an elution buffer. Anti-CA8 Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Mouse Carbonic Anhydrase VIII / CA8 protein (Catalog#50767-M08E)
Species Reactivity
Mouse Carbonic Anhydrase VIII
Source
Polyclonal Mouse Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Mouse Carbonic Anhydrase VIII / CA8 (rh Carbonic Anhydrase VIII / CA8; Catalog#50767-M08E; P28651; Met1-Gln291). Carbonic Anhydrase VIII / CA8 specific IgG was purified by Mouse Carbonic Anhydrase VIII / CA8 affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-CA8 Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-AW546993ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-Ca8ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-CalsALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-Cals1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-Car8ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-CarpALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-RP23-180H12.1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-wdlALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
CA8 Background Information
The carbonic anhydrases (or carbonate dehydratases) are classified as metalloenzyme for its zinc ion prosthetic group and form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons, a reversible reaction that takes part in maintaining acid-base balance in blood and other tissues. The carbonic anhydrasekl (CA) family consists of at least 11 enzymatically active members and a few inactive homologous proteins. Carbonic anhydrase protein (CA) VIII, which is a member of the CA gene family, has been shown to have no catalytic CA activity and its biological function is still unknown. Increased expression of CA-RP VIII was observed in 78% of colorectal carcinomas. It suggested that CA-RP VIII plays a role in the process of invasion in colorectal cancer.
Full Name
carbonic anhydrase VIII
References
Lehtonen J, et al. (2004) Characterization of CA XIII, a Novel Member of the Carbonic Anhydrase Isozyme Family. The Journal of Biological Chemistry. 279: 2719-27. Lindskog S. (1997) Structure and mechanism of carbonic anhydrase. Pharmacology & Therapeutics. 74(1)：1-20. Miyaji E, et al. (2003) Overexpression of carbonic anhydrase-related protein VIII in human colorectal cancer. The Journal of Pathology. 201(1): 37-45.
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