Anti-Syndecan-4 Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-Syndecan-4 Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-Syndecan-4 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-Syndecan-4 Magnetic Beads-IP Kit Product Description
The Anti-Syndecan-4 magnetic Beads, conjugated with Anti-Syndecan-4 antibody, are used for immuneprecipitation (IP) of Syndecan-4 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing Syndecan-4 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound Syndecan-4 proteins are dissociated from the magnetic beads using an elution buffer. Anti-Syndecan-4 Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Mouse Syndecan-4 / SDC4 protein (Catalog#50726-M08H)
Species Reactivity
Mouse Syndecan-4 / SDC4
Source
Monoclonal Mouse Rabbit IgG
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Mouse Syndecan-4 / SDC4 (rM Syndecan-4 / SDC4; Catalog#50726-M08H; O35988; Met1-Val146).
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-Syndecan-4 Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-AA959608ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-AW108331ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-ryudocanALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-Synd4ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-syndecan-4ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
Syndecan-4 Background Information
SDC4 (Syndecan-4), also known as Syn4, is a transmembrane heparan sulfate proteoglycan that co-operates with integrins during cell-matrix interactions for the assembly of focal adhesions and actin stress fibers and in the phosphorylation of focal adhesion kinase (FAK) on Tyr397. Syndecan-4 plays roles in the formation of focal adhesions and stress fibers. The cytoplasmic domain of syndecan-4 interacts with a number of signalling and structural proteins, and both extracellular and cytoplasmic domains are necessary for regulated activation of associated transmembrane receptors. Syndecan-4/SDC4 is a heparan sulfate proteoglycan and works as a coreceptor for various growth factors. SDC4 deficiency limits neointimal formation after vascular injury by regulating vascular smooth muscle cells (VSMCs) proliferation and vascular progenitor cells (VPCs) mobilization. Therefore, SDC4 may be a novel therapeutic target for preventing arterial restenosis after angioplasty.
References
Ikesue M, et al. (2011) Syndecan-4 deficiency limits neointimal formation after vascular injury by regulating vascular smooth muscle cell proliferation and vascular progenitor cell mobilization. Arterioscler Thromb Vasc Biol. 31(5): 1066-74. Saoncella S, et al. (2004) Syndecan-4 regulates ATF-2 transcriptional activity in a Rac1-dependent manner. J Biol Chem. 279(45): 47172-6. Bass MD, et al. (2002) Cytoplasmic interactions of syndecan-4 orchestrate adhesion receptor and growth factor receptor signalling. Biochem J. 368(Pt 1): 1-15. Couchman JR, et al. (1999) Syndecan-4 and integrins: combinatorial signaling in cell adhesion. J Cell Sci. 112 ( Pt 20): 3415-20.
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