Anti-EMAP-II Magnetic Beads-IP Kit Product Components
Components | Storage |
Anti-EMAP-II Magnetic Beads1,3 | 2-8℃ for 12 months |
NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
5×TBST(pH7.4) | |
1×TBST(pH7.4) | |
ddH2O | |
CD166 Positive Cell Lysate | -20℃ for 12 months |
Alkaline Elution Buffer | 2-8℃ for 12 months |
Acidity Elution Buffer | 2-8℃ for 12 months |
Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-EMAP-II magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-EMAP-II Magnetic Beads-IP Kit Product Description
The Anti-EMAP-II magnetic Beads, conjugated with Anti-EMAP-II antibody, are used for immuneprecipitation (IP) of EMAP-II proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing EMAP-II proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound EMAP-II proteins are dissociated from the magnetic beads using an elution buffer. Anti-EMAP-II Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Mouse AIMP1 / EMAP II protein (Catalog#50697-MNAE)
Species Reactivity
Mouse AIMP1 / EMAP II
Source
Polyclonal Mouse Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Mouse AIMP1 / EMAP II (rh AIMP1 / EMAP II; Catalog#50697-MNAE; Q62386; Ser145-Lys310). AIMP1 / EMAP II specific IgG was purified by Mouse AIMP1 / EMAP II affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-EMAP-II Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-9830137A06RikALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-AIMP1/p43ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-Emap2ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-EMAPIIALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-p43ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-Scye1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
EMAP-II Background Information
Aminoacyl tRNA synthase complex-interacting multifunctional protein 1, also known as Multisynthase complex auxiliary component p43, Endothelial monocyte-activating polypeptide II, AIMP1, EMAP2 and SCYE1, is a nucleus protein which contains one tRNA-binding domain. AIMP1 (also known as p43) is a factor associated with a macromolecular aminoacyl-tRNA synthetase (ARS) complex but also plays diverse regulatory roles in various physiological processes. AIMP1 negatively regulates TGF-beta signaling via stabilization of Smurf2. It suggests the novel activity of AIMP1 as a component of negative feedback loop of TGF-beta signaling. Recently, it been demonstrated that AIMP1 is also secreted and acts as a novel pleiotropic cytokine. AIMP1 protein induces the maturation and activation of DCs, which skew the immune response toward a Th1 response. AIMP1 is known as a cytokine working in the control of angiogenesis, inflammation, and wound healing. AIMP1 is secreted from the pancreas upon glucose starvation, and it also plays a glucagon-like role in glucose homeostasis. Although AIMP1 was identified as a component of the macromolecular aminoacyl tRNA synthetase complex involved in the cellular translation process, it was also found to be secreted as a cytokine having complex physiological functions. Among these, AIMP1's angiostatic and immune stimulating activities suggest its potential use as a novel antitumor therapeutic protein. AIMP1 may exert its antitumor activity by inducing tumor-suppressing cytokines. Thus, AIMP1 may be useful as a novel anti-tumor agent.
Full Name
aminoacyl tRNA synthetase complex-interacting multifunctional protein 1
References
Lee YS, et al. (2006) Antitumor activity of the novel human cytokine AIMP1 in an in vivo tumor model. Mol Cells. 21(2): 213-7. Park SG, et al. (2006) Hormonal activity of AIMP1/p43 for glucose homeostasis. Proc Natl Acad Sci U S A. 103(40): 14913-8. Kim E, et al. (2008) AIMP1/p43 protein induces the maturation of bone marrow-derived dendritic cells with T helper type 1-polarizing ability. J Immunol. 180(5): 2894-902. Lee YS, et al. (2008) AIMP1/p43 downregulates TGF-beta signaling via stabilization of smurf2. Biochem Biophys Res Commun. 371(3): 395-400. Han JM, et al. (2010) Antitumor activity and pharmacokinetic properties of ARS-interacting multi-functional protein 1 (AIMP1/p43). Cancer Lett. 287(2): 157-64.