Anti-Carbonic anhydrase II Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-Carbonic anhydrase II Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-Carbonic anhydrase II magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-Carbonic anhydrase II Magnetic Beads-IP Kit Product Description
The Anti-Carbonic anhydrase II magnetic Beads, conjugated with Anti-Carbonic anhydrase II antibody, are used for immuneprecipitation (IP) of Carbonic anhydrase II proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing Carbonic anhydrase II proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound Carbonic anhydrase II proteins are dissociated from the magnetic beads using an elution buffer. Anti-Carbonic anhydrase II Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Mouse Carbonic Anhydrase II / CA2 protein (Catalog#50685-M08E)
Species Reactivity
Mouse Carbonic Anhydrase II /
Source
Polyclonal Mouse Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Mouse Carbonic Anhydrase II / CA2 (rM Carbonic Anhydrase II / CA2; Catalog#50685-M08E; AAH55291.1; Met 1-Lys 260). Carbonic Anhydrase II / CA2 specific IgG was purified by Mouse Carbonic Anhydrase II / CA2 affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-Carbonic anhydrase II Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-AI131712ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-Ca2ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-CAIIALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-Car-2ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-Ltw-5ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-Lvtw-5ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
Carbonic anhydrase II Background Information
The carbonic anhydrases (or carbonate dehydratases) are classified as metalloenzyme for its zinc ion prosthetic group and form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons, a reversible reaction that takes part in maintaining acid-base balance in blood and other tissues. The carbonic anhydrasekl (CA) family consists of at least 11 enzymatically active members and a few inactive homologous proteins. Carbonic anhydrase II is one of fourteen forms of human α carbonic anhydrases. Defects in this enzyme are associated with osteopetrosis and renal tubular acidosis. Renal carbonic anhydrase allows the reabsorption of sodium ions in the proximal tubule. Carbonic anhydrase II has been shown to interact with Band 3 and Sodium-hydrogen antiporter 1.
Full Name
carbonic anhydrase II
References
Lehtonen J, et al. (2004) Characterization of CA XIII, a Novel Member of the Carbonic Anhydrase Isozyme Family. The Journal of Biological Chemistry. 279: 2719-27. Lindskog S. (1997) Structure and mechanism of carbonic anhydrase. Pharmacology & Therapeutics. 74(1)：1-20． Lilias A, et al. (1972) Crystal Structure of Human Carbonic Anhydrase C. Nature new biology. 235: 131-7. Li XJ, et al. (2002) Carbonic Anhydrase II Binds to and Enhances Activity of the Na+/H+ Exchanger. The Journal of Biological Chemistry. 277: 36085-91.
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