Anti-Argonaute-2 Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-Argonaute-2 Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-Argonaute-2 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-Argonaute-2 Magnetic Beads-IP Kit Product Description
The Anti-Argonaute-2 magnetic Beads, conjugated with Anti-Argonaute-2 antibody, are used for immuneprecipitation (IP) of Argonaute-2 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing Argonaute-2 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound Argonaute-2 proteins are dissociated from the magnetic beads using an elution buffer. Anti-Argonaute-2 Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Mouse AGO2 protein (Catalog#50683-M07B)
Species Reactivity
Mouse AGO2
Source
Monoclonal Mouse Rabbit IgG
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Mouse AGO2 (rM AGO2; Catalog#50683-M07B; Q8CJG0; Met1-Ala860).
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-Argonaute-2 Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-1110029L17RikALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-2310051F07RikALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-AI225898ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-AL022874ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-AW546247ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-Eif2c2ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-ENSMUSG00000072493ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-Gerp95ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-Gm10365ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-mKIAA4215ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
Argonaute-2 Background Information
Argonaute 2 (AGO2), also known as Eukaryotic translation initiation factor 2C2 (EIF2C2), belongs to the Argonaute family, AGO subfamily, which is a component of the RNA-induced silencing complex (RISC) and mediates small interfering RNA (siRNA)-directed mRNA cleavage and microRNA translational suppression. AGO2 protein is the catalytic engine of mammalian RNAi. It contains a PIWI domain that is structurally related to RNases H and possibly shares with them a two-metal-ion catalysis mechanism. Human AGO2 was unable to cleave preformed RNA duplexes and exhibited weaker binding affinity for RNA duplexes compared with the single strand RNA. The enzyme exhibited greater RNase H activity in the presence of Mn2+ compared with Mg2+. Human AGO2 exhibited weaker binding affinities and reduced cleavage activities for antisense RNAs with either a 5'-terminal hydroxyl or abasic nucleotide. In mouse hematopoiesis, AGO2 controls early development of lymphoid and erythroid cells. AGO2 is a highly specialized member of the Argonaute family with an essential nonredundant Slicer-independent function within the mammalian miRNA pathway. AGO2 regulates dFMR1 expression, and the relationship between dFMR1 and AGO2 was defined by their physical interaction and co-regulation of downstream targets. AGO2 and dFMR1 are also connected through a regulatory relationship. AGO2 is a regulator of dFMR1 expression and have clarified an important developmental role for AGO2 in the nervous system and germ line that requires dFMR1 function. In addition, AGO2 is regulated at both the transcriptional and posttranslational level, and also implicate AGO2 and enhanced micro-RNA activity in the tumorigenic progression of breast cancer cell lines.
Full Name
argonaute RISC catalytic component 2
References
O'Carroll D, et al. (2007) A Slicer-independent role for Argonaute 2 in hematopoiesis and the microRNA pathway. Genes Dev. 21(16): 1999-2004. Pepper AS, et al. (2009) Argonaute2 suppresses Drosophila fragile X expression preventing neurogenesis and oogenesis defects. PLoS One. 4(10): e7618. Lima WF, et al. (2009) Binding and cleavage specificities of human Argonaute2. J Biol Chem. 284(38): 26017-28. Adams BD, et al. (2009) Argonaute-2 expression is regulated by epidermal growth factor receptor and mitogen-activated protein kinase signaling and correlates with a transformed phenotype in breast cancer cells. Endocrinology. 150(1): 14-23. Salvatore V, et al. (2010) Bacterial expression of mouse argonaute 2 for functional and mutational studies. Int J Mol Sci. 11(2): 745-53. Wilson JA, et al. (2011) Human Ago2 is required for efficient microRNA 122 regulation of hepatitis C virus RNA accumulation and translation. J Virol. 85(5): 2342-50.
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