Anti-E-Cadherin Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-E-Cadherin Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-E-Cadherin magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-E-Cadherin Magnetic Beads-IP Kit Product Description
The Anti-E-Cadherin magnetic Beads, conjugated with Anti-E-Cadherin antibody, are used for immuneprecipitation (IP) of E-Cadherin proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing E-Cadherin proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound E-Cadherin proteins are dissociated from the magnetic beads using an elution buffer. Anti-E-Cadherin Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Mouse E-Cadherin / CDH1 / E-cad / CD324 protein (Catalog#50671-M08H)
Species Reactivity
Mouse E-Cadherin / CDH1 / E-ca
Source
Polyclonal Mouse Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Mouse E-Cadherin / CDH1 / E-cad / CD324 (rh E-Cadherin / CDH1 / E-cad / CD324; Catalog#50671-M08H; P09803; Met1-Val709). E-Cadherin / CDH1 / E-cad / CD324 specific IgG was purified by Mouse E-Cadherin / CDH1 / E-cad / CD324 affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-E-Cadherin Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-AA960649ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-ARC-1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-E-cadALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-EcadALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-L-CAMALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-UmALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-UVOALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
E-Cadherin Background Information
Cadherins are calcium-dependent cell adhesion proteins which preferentially interact with themselves in a homophilic manner in connecting cells, and thus may contribute to the sorting of heterogeneous cell type. E-cadherin (E-Cad), also known as CDH1 and CD324, is a calcium-dependent cell adhesion molecule the intact function of which is crucial for the establishment and maintenance of epithelial tissue polarity and structural integrity. Mutations in CDH1 occur in diffuse type gastric cancer, lobular breast cancer, and endometrial cancer. In human cancers, partial or complete loss of E-cadherin expression correlates with malignancy. During apoptosis or with calcium influx, E-Cad is cleaved by the metalloproteinase to produce fragments of about 38 kDa (E-CAD/CTF1), 33 kDa (E-CAD/CTF2) and 29 kDa (E-CAD/CTF3), respectively. E-Cad has been identified as a potent invasive suppressor, as downregulation of E-cadherin expression is involved in dysfunction of the cell-cell adhesion system, and often correlates with strong invasive potential and poor prognosis of human carcinomas.
Full Name
cadherin 1, type 1, E-cadherin (epithelial)
References
Wang HD, et al. (2004) CDH1 germline mutation in hereditary gastric carcinoma. World J Gastroenterol. 10(21): 3088-93. Masterson J, et al. (2007) Posttranslational truncation of E-cadherin and significance for tumour progression. Cells Tissues Organs. 185(1-3): 175-9. Mrgineanu E, et al. (2008) Correlation between E-cadherin abnormal expressions in different types of cancer and the process of metastasis. Rev Med Chir Soc Med Nat Iasi. 112(2): 432-6.
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