Anti-P4HB Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-P4HB Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-P4HB magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-P4HB Magnetic Beads-IP Kit Product Description
The Anti-P4HB magnetic Beads, conjugated with Anti-P4HB antibody, are used for immuneprecipitation (IP) of P4HB proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing P4HB proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound P4HB proteins are dissociated from the magnetic beads using an elution buffer. Anti-P4HB Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Mouse P4HB protein (Catalog#50638-M08H)
Species Reactivity
Mouse P4HB
Source
Polyclonal Mouse Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Mouse P4HB (rM P4HB; Catalog#50638-M08H; NP_035162.1; Met 1-Lys 506). P4HB specific IgG was purified by Mouse P4HB affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-P4HB Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-ERp59ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-PDIALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-Pdia1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-ThbpALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
P4HB Background Information
Protein disulfide-isomerase, also known as Cellular thyroid hormone-binding protein, Prolyl 4-hydroxylase subunit beta, p55 and P4HB, is a peripheral membrane protein which belongs to the protein disulfide isomerase family. P4HB is highly abundant. In some cell types, it seems to be also secreted or associated with the plasma membrane, where it undergoes constant shedding and replacement from intracellular sources. P4HB localizes near CD4-enriched regions on lymphoid cell surfaces. It is identified by mass spectrometry in melanosome fractions from stage I to stage IV. P4HB reduces and may activate fusogenic properties of HIV-1 gp12 surface protein, thereby enabling HIV-1 entry into the cell. P4HB catalyzes the formation, breakage and rearrangement of disulfide bonds. At the cell surface, it seems to act as a reductase that cleaves disulfide bonds of proteins attached to the cell. P4HB may therefore cause structural modifications of exofacial proteins. Inside the cell, it seems to form/rearrange disulfide bonds of nascent proteins. At high concentrations, P4HB functions as a chaperone that inhibits aggregation of misfolded proteins. At low concentrations, it facilitates aggregation (anti-chaperone activity). P4HB may be involved with other chaperones in the structural modification of the TG precursor in hormone biogenesis. It also acts a structural subunit of various enzymes such as prolyl 4-hydroxylase and microsomal triacylglycerol transfer protein MTTP.
Full Name
prolyl 4-hydroxylase, beta polypeptide
References
Kivirikko KI, et al., 1989, FASEB J., 3 (5): 1609-17. Pihlajaniemi T, et al.,1991, J Hepatol., 13, Suppl 3: S2 Fenouillet E., et al., 2001, J. Infect. Dis. 183:744-752. Gevaert K., et al., 2003, Nat. Biotechnol. 21:566-569. Barbouche R., et al., 2003, J. Biol. Chem. 278:3131-3136.
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