Anti-HSP60 Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-HSP60 Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-HSP60 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-HSP60 Magnetic Beads-IP Kit Product Description
The Anti-HSP60 magnetic Beads, conjugated with Anti-HSP60 antibody, are used for immuneprecipitation (IP) of HSP60 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing HSP60 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound HSP60 proteins are dissociated from the magnetic beads using an elution buffer. Anti-HSP60 Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Mouse HSPD1 / HSP60 Protein (Catalog#50596-M07E)
Species Reactivity
Mouse HSPD1 / HSP60
Source
Polyclonal Mouse Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Mouse HSPD1 / HSP60 (rM HSPD1 / HSP60; Catalog#50596-M07E; NP_034607.3; Leu2-Phe573). HSPD1 / HSP60 specific IgG was purified by Mouse HSPD1 / HSP60 affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-HSP60 Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-60kDaALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-Hsp60ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
HSP60 Background Information
HSPD1, also known as HSP6, is a member of the chaperonin family. HSPD1 may function as a signaling molecule in the innate immune system. This protein is essential for the folding and assembly of newly imported proteins in the mitochondria. It may also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix. HSPD1 gene is adjacent to a related family member and the region between the 2 genes functions as a bidirectional promoter. Several pseudogenes have been associated with this gene. Mutations associated with this gene cause autosomal recessive spastic paraplegia 13.Defects in HSPD1 are a cause of spastic paraplegia autosomal dominant type 13 (SPG13). Spastic paraplegia is a degenerative spinal cord disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs. Defects in HSPD1 are the cause of leukodystrophy hypomyelinating type 4 (HLD4); also called mitochondrial HSP6 chaperonopathy or MitCHAP-6 disease. HLD4 is a severe autosomal recessive hypomyelinating leukodystrophy. HSPD1 is cinically characterized by infantile-onset rotary nystagmus, progressive spastic paraplegia, neurologic regression, motor impairment, profound mental retardation. Death usually occurrs within the first two decades of life.
Immune Checkpoint Immunotherapy Cancer Immunotherapy Targeted Therapy Full Name
heat shock 60kDa protein 1 (chaperonin)
References
Hansen J J, et al. (2002) Hereditary spastic paraplegia SPG13 is associated with a mutation in the gene encoding the mitochondrial chaperonin Hsp60. Am J Hum Genet. 70: 1328-32. Magen D, et al. (2008) Mitochondrial Hsp60 chaperonopathy causes an autosomal-recessive neurodegenerative disorder linked to brain hypomyelination and leukodystrophy. Am J Hum Genet. 83: 30-42. Venner TJ, et al. (1990) Nucleotide sequences and novel structural features of human and Chinese hamster hsp60 (chaperonin) gene families. DNA Cell Biol. 9 (8): 545-52.
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