Anti-14-3-3 sigma Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-14-3-3 sigma Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-14-3-3 sigma magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-14-3-3 sigma Magnetic Beads-IP Kit Product Description
The Anti-14-3-3 sigma magnetic Beads, conjugated with Anti-14-3-3 sigma antibody, are used for immuneprecipitation (IP) of 14-3-3 sigma proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing 14-3-3 sigma proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound 14-3-3 sigma proteins are dissociated from the magnetic beads using an elution buffer. Anti-14-3-3 sigma Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Mouse 14-3-3 sigma / Stratifin / YWHAS / SFN protein (Catalog#50576-MNCE)
Species Reactivity
Mouse 14-3-3 sigma / Stratifin
Source
Polyclonal Mouse Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Mouse 14-3-3 sigma / Stratifin / YWHAS / SFN (rM 14-3-3 sigma / Stratifin / YWHAS / SFN; Catalog#50576-MNCE; NP_061224.2; Met 1-Ser 248). 14-3-3 sigma / Stratifin / YWHAS / SFN specific IgG was purified by Mouse 14-3-3 sigma / Stratifin / YWHAS / SFN affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-14-3-3 sigma Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-ErALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-Mme1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-YwhasALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
14-3-3 sigma Background Information
14-3-3 protein sigma (YWHAS), also known as stratifin (SFN) and epithelial cell marker protein 1, is a member of the14-3-3 proteins which are a family of conserved regulatory molecules expressed in all eukaryotic cells. The name 14-3-3 refers to the particular elution and migration pattern of these proteins on DEAE-cellulose chromatography and starch-gel electrophoresis. The 14-3-3 proteins eluted in the 14th fraction of bovine brain homogenate and were found on positions 3.3 of subsequent electrophoresis. There are seven genes that encode 14-3-3s in most mammals. 14-3-3 proteins have been identified as adapter proteins implicated in the regulation of a large spectrum of both general and specialized signaling pathway. More than 1 signaling proteins have been reported as 14-3-3 ligands including kinases, phosphatases, and transmembrane receptors, and the binding generally results in the modulation of the activity of the binding partner. YWHAE exists as a homodimer and present mainly in tissues enriched in stratified squamous keratinising epithelium. YWHAS has been repoted to interact with KRT17 and GAB2, and may regulate protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway upon binding to KRT17. Additionally, YWHAS (SFN) may also act as a p53-regulated inhibitor of G2/M progression.
References
Zakut-Houri R., et al.,(1985), Human p53 cellular tumor antigen: cDNA sequence and expression in COS cells. EMBO J. 4:1251-1255. Lamb P., et al., (1986), Characterization of the human p53 gene.Mol. Cell. Biol. 6:1379-1385. Harlow E., et al.,(1985), Molecular cloning and in vitro expression of a cDNA clone for human cellular tumor antigen p53.Mol. Cell. Biol. 5:1601-1610.
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