Anti-CSRP1 Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-CSRP1 Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-CSRP1 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-CSRP1 Magnetic Beads-IP Kit Product Description
The Anti-CSRP1 magnetic Beads, conjugated with Anti-CSRP1 antibody, are used for immuneprecipitation (IP) of CSRP1 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing CSRP1 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound CSRP1 proteins are dissociated from the magnetic beads using an elution buffer. Anti-CSRP1 Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Mouse CSRP1 protein (Catalog#50532-M08E)
Species Reactivity
Mouse CSRP1
Source
Polyclonal Mouse Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Mouse CSRP1 (rh CSRP1; Catalog#50532-M08E; P97315; Met1-Glu193). CSRP1 specific IgG was purified by Mouse CSRP1 affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-CSRP1 Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-AA408841ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-AA959891ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-AW545626ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-CRP1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-CsrpALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
CSRP1 Background Information
Cysteine and glycine-rich protein 1, also known as Cysteine-rich protein 1, CSRP1 and CSRP, is a member of the CSRP family which may be involved in regulatory processes important for development and cellular differentiation. CSRP1 contains two LIM zinc-binding domains. The LIM / double zinc-finger motif found in CSRP1 is found in a group of proteins with critical functions in gene regulation, cell growth, and somatic differentiation. Zebrafish CSRP1 is expressed in the mesendoderm and its derivatives. CSRP1 interacts with Dishevelled 2 (Dvl2) and Diversin (Div), which control cell morphology and other dynamic cell behaviors via the noncanonical Wnt and JNK pathways. When CSRP1 message is knocked down, abnormal convergent extension cell movement is induced, resulting in severe deformities in midline structures. In addition, cardiac bifida is induced as a consequence of defects in cardiac mesoderm cell migration. CSRP1 acts as a key molecule of the noncanonical Wnt pathway, which orchestrates cell behaviors during dynamic morphogenetic movements of tissues and organs.
Full Name
cysteine and glycine-rich protein 1
References
Wimmer,U. et al., 2005, Nucleic acids Res. 33 (18):5715-27. Miyasaka, KY.et al., 2007, Proc Natl Acad Sci USA. 104 (27): 11274-9. Zhou,C.Z. et al., 2008,Chin Med J. 121 (24): 2479-86. Lilly,B. et al., 2010, Arterioscler Thromb Vasc Biol. 30 (4):694-701.
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