Anti-15-PGDH Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-15-PGDH Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-15-PGDH magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-15-PGDH Magnetic Beads-IP Kit Product Description
The Anti-15-PGDH magnetic Beads, conjugated with Anti-15-PGDH antibody, are used for immuneprecipitation (IP) of 15-PGDH proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing 15-PGDH proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound 15-PGDH proteins are dissociated from the magnetic beads using an elution buffer. Anti-15-PGDH Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Mouse HPGD / 15-PGDH protein (Catalog#50531-M08E)
Species Reactivity
Mouse HPGD / 15-PGDH
Source
Monoclonal Mouse Rabbit IgG
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Mouse HPGD / 15-PGDH (rM HPGD / 15-PGDH; Catalog#50531-M08E; Q8VCC1; Met1-Ser269).
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-15-PGDH Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-15-PGDHALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-AV026552ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
15-PGDH Background Information
15-hydroxyprostaglandin dehydrogenase [NAD+], also known as Prostaglandin dehydrogenase 1, HPGD, and PGDH1, is a member of the short-chain dehydrogenases/reductases (SDR) family. Prostaglandins (PGs) play a key role in the onset of labor in many species and regulate uterine contractility and cervical dilatation. Therefore, the regulation of prostaglandin output by PG synthesizing and metabolizing enzymes in the human myometrium may determine uterine activity patterns in human labor both at preterm and at term. Prostaglandin dehydrogenase (PGDH) metabolizes prostaglandins (PGs) to render them inactive. HPGD is down-regulated by cortisol, dexamethasone and betamethasone and down-regulated in colon cancer. It is up-regulated by TGFB1. HPGD contributes to the regulation of events that are under the control of prostaglandin levels. HPGD catalyzes the NAD-dependent dehydrogenation of lipoxin A4 to form 15-oxo-lipoxin A4. and inhibits in vivo proliferation of colon cancer cells. Defects in HPGD are the cause of primary hypertrophic osteoathropathy autosomal recessive (PHOAR) , cranioosteoarthropathy (COA), and isolated congenital nail clubbing.
Full Name
hydroxyprostaglandin dehydrogenase 15-(NAD)
References
Patel, FA. et al., 2003, J. Clin. Endocrinol. Metab. 88: 2922-33. McKeown KJ, et al.,2003, J. Clin. Endocrinol. Metab. 88 (4): 1737-41. Yan, M. et al., 2004, Proc. Natl. Acad. Sci. USA. 101: 17468-73. Tariq, M. et al., 2009, J Med Genet. 46 (1): 14-20.
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