Anti-methionyl aminopeptidase 2 Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-methionyl aminopeptidase 2 Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-methionyl aminopeptidase 2 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-methionyl aminopeptidase 2 Magnetic Beads-IP Kit Product Description
The Anti-methionyl aminopeptidase 2 magnetic Beads, conjugated with Anti-methionyl aminopeptidase 2 antibody, are used for immuneprecipitation (IP) of methionyl aminopeptidase 2 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing methionyl aminopeptidase 2 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound methionyl aminopeptidase 2 proteins are dissociated from the magnetic beads using an elution buffer. Anti-methionyl aminopeptidase 2 Magnetic Beads-IP Kit Antibody Information
Antibody
Anti-methionyl aminopeptidase 2 Antibody(
50528-RP02)
Immunogen
Recombinant Mouse METAP2 / MAP2 protein (Catalog#50528-M08B)
Species Reactivity
Mouse METAP2 / MAP2
Source
Polyclonal Mouse Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Mouse METAP2 / MAP2 (rM METAP2 / MAP2; Catalog#50528-M08B; O08663; Ala2-Tyr478). METAP2 / MAP2 specific IgG was purified by Mouse METAP2 / MAP2 affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-methionyl aminopeptidase 2 Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-4930584B20RikALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-A930035J23RikALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-AI047573ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-AL024412ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-Amp2ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-AU014659ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-MnpepALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-p67ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-p67eIF2ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
methionyl aminopeptidase 2 Background Information
METAP2 (Methionine aminopeptidase 2), also known as MAP2 is a a protein which belongs to the peptidase M24A family. MAP2 binds 2 cobalt or manganese ions and contains approximately 12 O-linked N-acetylglucosamine (GlcNAc) residues. It is found in all organisms and is especially important because of its critical role in tissue repair and protein degradation. The catalytic activity of human MAP2 toward Met-Val peptides is consistently two orders of magnitude higher than that of METAP1, suggesting that it is responsible for processing proteins containing N-terminal Met-Val and Met-Thr sequences in vivo. This protein functions both by protecting the alpha subunit of eukaryotic initiation factor 2 from inhibitory phosphorylation and by removing the amino-terminal methionine residue from nascent protein. MAP2 protects eukaryotic initiation factor EIF2S1 from translation-inhibiting phosphorylation by inhibitory kinases such as EIF2AK2/PKR and EIF2AK1/HCR. It also plays a critical role in the regulation of protein synthesis.
Full Name
methionyl aminopeptidase 2
References
Bennett, et al. (1997) EPR Studies on the Mono- and Dicobalt (II)-Substituted Forms of the Aminopeptidase from Aeromonas proteolytica. Insight into the Catalytic Mechanism of Dinuclear Hydrolases. J Am Chem Soc. 119:1923-33. Johansson, et al. (2008) Dicobalt II-II, II-III, and III-III Complexes as Spectroscopic Models for Dicobalt Enzyme Active Sites. Inorg Chem. 47:5079-92. Bradshaw, et al. (2002) Aminopeptidases and angiogenesis. Essays Biochem. 38: 5-78.
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