Anti-CTLA-4 Magnetic Beads-IP Kit Product Components
Components | Storage |
Anti-CTLA-4 Magnetic Beads1,3 | 2-8℃ for 12 months |
NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
5×TBST(pH7.4) | |
1×TBST(pH7.4) | |
ddH2O | |
CD166 Positive Cell Lysate | -20℃ for 12 months |
Alkaline Elution Buffer | 2-8℃ for 12 months |
Acidity Elution Buffer | 2-8℃ for 12 months |
Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-CTLA-4 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-CTLA-4 Magnetic Beads-IP Kit Product Description
The Anti-CTLA-4 magnetic Beads, conjugated with Anti-CTLA-4 antibody, are used for immuneprecipitation (IP) of CTLA-4 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing CTLA-4 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound CTLA-4 proteins are dissociated from the magnetic beads using an elution buffer. Anti-CTLA-4 Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Mouse CTLA-4 / CD152 protein (Catalog#50503-M08H)
Species Reactivity
Mouse CTLA-4 / CD152
Source
Polyclonal Mouse Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Mouse CTLA-4 / CD152 (rM CTLA-4 / CD152; Catalog#50503-M08H; NP_033973.2; Met 1-Phe 162). CTLA-4 / CD152 specific IgG was purified by Mouse CTLA-4 / CD152 affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-CTLA-4 Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-Cd152ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-Ctla-4ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-Ly-56ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
CTLA-4 Background Information
Full Name
cytotoxic T-lymphocyte-associated protein 4
References
Slavik JM, et al. (1999) CD28/CTLA-4 and CD80/CD86 families: signaling and function. Immunol Res. 19(1): 1-24. Holmberg D, et al. (2005) CTLA-4 (CD152) and its involvement in autoimmune disease. Autoimmunity. 38(3): 225-33. Chin LT, et al. (2008) Immune intervention with monoclonal antibodies targeting CD152 (CTLA-4) for autoimmune and malignant diseases. Chang Gung Med J. 31(1): 1-15.