Anti-CHEK2 Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-CHEK2 Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-CHEK2 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-CHEK2 Magnetic Beads-IP Kit Product Description
The Anti-CHEK2 magnetic Beads, conjugated with Anti-CHEK2 antibody, are used for immuneprecipitation (IP) of CHEK2 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing CHEK2 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound CHEK2 proteins are dissociated from the magnetic beads using an elution buffer. Anti-CHEK2 Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Mouse CHK2 / CHEK2 protein (Catalog#50432-M20B)
Species Reactivity
Mouse CHK2 / CHEK2
Source
Polyclonal Mouse Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Mouse CHK2 / CHEK2 (rM CHK2 / CHEK2; Catalog#50432-M20B; Q9Z265; Mey1-Leu 546). CHK2 / CHEK2 specific IgG was purified by Mouse CHK2 / CHEK2 affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-CHEK2 Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-Cds1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-CHK2ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-HUCDS1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-Rad53ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
CHEK2 Background Information
In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by CHEK2 gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded CHEK2 protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in CHEK2s gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Several transcript variants encoding different isoforms have been found for this gene.
Immune Checkpoint Immunotherapy Cancer Immunotherapy Targeted Therapy Full Name
checkpoint kinase 2
References
Bogdanova N, et al. (2005) Association of two mutations in the CHEK2 gene with breast cancer. Cancer Genetics. 116(2) : 263-6. Dong XY, et al. (2003) Mutations in CHEK2 associated with prostate cancer risk. The American journal of human genetics. 72(2) 270-80. Massink MPG, Kooi IE, Martens JWM, Waisfisz Q, Meijers-Heijboer H. Genomic profiling of CHEK2*1100delC-mutated breast carcinomas. BMC Cancer. 2015;15:877. doi:10.1186/s12885-015-1880-y.
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