Anti-alanyl-tRNA synthetase Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-alanyl-tRNA synthetase Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-alanyl-tRNA synthetase magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-alanyl-tRNA synthetase Magnetic Beads-IP Kit Product Description
The Anti-alanyl-tRNA synthetase magnetic Beads, conjugated with Anti-alanyl-tRNA synthetase antibody, are used for immuneprecipitation (IP) of alanyl-tRNA synthetase proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing alanyl-tRNA synthetase proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound alanyl-tRNA synthetase proteins are dissociated from the magnetic beads using an elution buffer. Anti-alanyl-tRNA synthetase Magnetic Beads-IP Kit Antibody Information
Antibody
Anti-alanyl-tRNA synthetase Antibody(
50384-T60)
Immunogen
Recombinant Mouse AARS / alanyl-tRNA synthetase Protein (Catalog#50384-M08B)
Species Reactivity
Mouse AARS / alanyl-tRNA synth
Source
Polyclonal Mouse Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Mouse AARS / alanyl-tRNA synthetase (rM AARS / alanyl-tRNA synthetase; Catalog#50384-M08B; Q8BGQ7; Met1-Asn968). AARS / alanyl-tRNA synthetase specific IgG was purified by Mouse AARS / alanyl-tRNA synthetase affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-alanyl-tRNA synthetase Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-AI316495ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-C76919ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-stiALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
alanyl-tRNA synthetase Background Information
Alanyl-tRNA synthetase (AARS) belongs to the family of ligases, specifically those forming carbon-oxygen bonds in aminoacyl-tRNA and related compounds. This enzyme participates in alanine and aspartate metabolism and aminoacyl-tRNA biosynthesis. Alanyl-tRNA synthetase (AlaRS) catalyzes synthesis of Ala-tRNA (Ala) and hydrolysis of mis-acylated Ser- and Gly-tRNA (Ala) at 2 different catalytic sites. Their role is not confined to catalyze the attachment of amino acids to transfer RNAs and thereby establish the rules of genetic code by virtue of matching the nucleotide triplet of anticodon with cognate amino acid. Under apoptotic conditions in cell culture, the full-length enzyme is secreted, and the two cytokine activities can be generated by leukocyte elastase, an extracellular protease. Secretion of this tRNA synthetase may contribute to apoptosis both by arresting translation and producing needed cytokines. This protein could be an attractive target of drugs against bacterial, fungal and parasitic infections.
Full Name
alanyl-tRNA synthetase
References
Wakasugi K, et al. (1999) Two Distinct Cytokines Released from a Human Aminoacyl-tRNA Synthetase. Science. 284 (5411): 147-51. Sokabe M, et al. (2009) The structure of alanyl-tRNA synthetase with editing domain. Proc Natl Acad Sci . 106 (27): 11028-33. Skupinska M, et al. (2009) AARS--the etiological factor and the attractive target of many disorders. Postepy Biochem. 55 (4): 373-84.
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