Anti-GLO1 Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-GLO1 Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-GLO1 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-GLO1 Magnetic Beads-IP Kit Product Description
The Anti-GLO1 magnetic Beads, conjugated with Anti-GLO1 antibody, are used for immuneprecipitation (IP) of GLO1 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing GLO1 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound GLO1 proteins are dissociated from the magnetic beads using an elution buffer. Anti-GLO1 Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Mouse GLO1 protein (Catalog#50364-M07E)
Species Reactivity
Mouse Glyoxalase I / GLO1
Source
Polyclonal Mouse Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Mouse GLO1 (rM GLO1; Catalog#50364-M07E; NP_079650.3; Ala 2-Ile 184). GLO1 specific IgG was purified by mouse GLO1 affinity chromatography .
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-GLO1 Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-0610009E22RikALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-1110008E19RikALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-2510049H23RikALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-AW550643ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-Glo-1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-Glo-1rALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-Glo-1sALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-Glo1-rALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-Glo1-sALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-GLY1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-QgloALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
GLO1 Background Information
Lactoylglutathione lyase, also known as Methylglyoxalase, Aldoketomutase, Glyoxalase I, Ketone-aldehyde mutase, S-D-lactoylglutathione methylglyoxal lyase and GLO1, is a member of the glyoxalase I family. GLO1 / Glyoxalase I is a ubiquitous cellular defense enzyme involved in the detoxification of methylglyoxal, a cytotoxic byproduct of glycolysis. Accumulative evidence suggests an important role of GLO1 expression in protection against methylglyoxal-dependent protein adduction and cellular damage associated with diabetes, cancer, and chronological aging. GLO1 / Glyoxalase I has been implicated in anxiety-like behavior in mice and in multiple psychiatric diseases in humans. GLO1 / Glyoxalase I catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione. GLO1 / Glyoxalase I exists in three separable isoforms which originate from two alleles in the genome. These correspond to two homodimers and one heterodimer composed of two subunits showing different electrophoretic properties. GLO1 upregulation may play a functional role in glycolytic adaptations of cancer cells.
References
Wu, YY. et al., 2008,Prog Neuropsychopharmacol Biol Psychiatry. 32 (7):1740-4. Williams,R. et al., 2009, PLoS One 4 (3): e4649. Antognelli,C. et al., 2009, BMC Cancer 9 :115. Bair,W.B. et al., 2010, Melanoma Res 20 (2):85-96.
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