Anti-Cathepsin A Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-Cathepsin A Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-Cathepsin A magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-Cathepsin A Magnetic Beads-IP Kit Product Description
The Anti-Cathepsin A magnetic Beads, conjugated with Anti-Cathepsin A antibody, are used for immuneprecipitation (IP) of Cathepsin A proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing Cathepsin A proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound Cathepsin A proteins are dissociated from the magnetic beads using an elution buffer. Anti-Cathepsin A Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Mouse Cathepsin A / CTSA protein (Catalog#50348-M08H)
Species Reactivity
Mouse Cathepsin A / CTSA
Source
Polyclonal Mouse Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Mouse Cathepsin A / CTSA (rh Cathepsin A / CTSA; Catalog#50348-M08H; P16675-1; Met1-Tyr474). Cathepsin A / CTSA specific IgG was purified by Mouse Cathepsin A / CTSA affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-Cathepsin A Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-AU019505ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-PPCAALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-PpgbALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
Cathepsin A Background Information
Lysosomal carboxypeptidase, cathepsin A (protective protein, CathA), is a component of the lysosomal multienzyme complex along with beta-galactosidase (GAL) and sialidase Neu1, where it activates Neu1 and protects GAL and Neu1 against the rapid proteolytic degradation. Cathepsin A is a multicatalytic enzyme with deamidase and esterase in addition to carboxypeptidase activities. It was recently identified in human platelets as deamidase. In vitro, it hydrolyzes a variety of bioactive peptide hormones including tachykinins, suggesting that extralysosomal cathepsin A plays a role in regulation of bioactive peptide functions. It is a member of the alpha/beta hydrolase fold family and has been suggested to share a common ancestral relationship with other alpha/beta hydrolase fold enzymes, such as cholinesterases. Cathepsin A defects are linked to multiple forms of Galactosialidosis with a combined secondary deficiency of beta-galactosidase and neuraminidase. Cathepsin A is a key molecule in the onset of galactosialidosis and also highlight the therapeutic acts in vivo as an endothelin-1-inactivating enzyme and strongly confirm a crucial role of this enzyme in effective elastic fiber formation.
References
Hiraiwa M. (1999) Cathepsin A/protective protein: an unusual lysosomal multifunctional protein. Cell Mol Life Sci. 56(11-12): 894-907. Yoshida T, et al. (2006) Comparative analysis of binding energy of chymostatin with human cathepsin A and its homologous proteins by molecular orbital calculation. J Chem Inf Model. 46(5): 2093-103. Seyrantepe V, et al. (2008) Enzymatic activity of lysosomal carboxypeptidase (cathepsin) A is required for proper elastic fiber formation and inactivation of endothelin-1. Circulation. 117(15): 1973-81.
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