Anti-CD157 Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-CD157 Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-CD157 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-CD157 Magnetic Beads-IP Kit Product Description
The Anti-CD157 magnetic Beads, conjugated with Anti-CD157 antibody, are used for immuneprecipitation (IP) of CD157 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing CD157 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound CD157 proteins are dissociated from the magnetic beads using an elution buffer. Anti-CD157 Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Mouse BST1 protein (Catalog#50319-M08H)
Species Reactivity
Mouse CD157 / BST1
Source
Polyclonal Mouse Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Mouse BST1 (rM BST1; Catalog#50319-M08H; NP_033893.2; Met 1-Glu 285). BST1 specific IgG was purified by mouse BST1 affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-CD157 Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-114/A10ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-A530073F09ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-BP-3ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-Bp3ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-Bsta1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-CD157ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-Ly65ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
CD157 Background Information
The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 32 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD157, also known as ADP-ribosyl cyclase 2, is an ectoenzyme sharing several characteristics with ADP-ribosyl cyclase CD38. CD157 was originally identified as a bone marrow stromal cell molecule (BST-1) with a glycosylphosphatidylinositol (GPI) anchor to bind to the cell surface. CD157 is prevalently expressed by cells of the myeloid lineage. CD157 could act as a receptor with signal transduction capability. Further, it regulates calcium homeostasis and promotes polarization in neutrophils and mediates superoxide (O 2?) production in the human U937 myeloid line.
Full Name
bone marrow stromal cell antigen 1
References
Zola H, et al. (2007) CD molecules 2006-human cell differentiation molecules. J Immunol Methods. 318 (1-2): 1-5. Ho IC, et al. (2009) GATA3 and the T-cell lineage: essential functions before and after T-helper-2-cell differentiation. Nat Rev Immunol. 9 (2): 125-35. Matesanz-Isabel J, et al. (2011) New B-cell CD molecules. Immunology Letters.134 (2): 104-12. Malavasi F, et al. (2006) CD38 and CD157 as Receptors of the Immune System: A Bridge Between Innate and Adaptive Immunity. Molecular Medicine. 12 (11-12): 334-41. Ortolan E, et al. (2002) CD157, the janus of CD38 but with a unique personality. Cell Biochemistry and Function. 20 (4): 309-22.
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