Anti-kynureninase Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-kynureninase Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-kynureninase magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-kynureninase Magnetic Beads-IP Kit Product Description
The Anti-kynureninase magnetic Beads, conjugated with Anti-kynureninase antibody, are used for immuneprecipitation (IP) of kynureninase proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing kynureninase proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound kynureninase proteins are dissociated from the magnetic beads using an elution buffer. Anti-kynureninase Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Mouse KYNU Protein (Catalog#50310-M08B)
Species Reactivity
Mouse KYNU
Source
Polyclonal Mouse Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Mouse KYNU (rM KYNU; Catalog#50310-M08B; Q9CXF0; Met1-Ser464). KYNU specific IgG was purified by Mouse KYNU affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-kynureninase Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-4432411A05RikALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
kynureninase Background Information
Genetic studies in mouse and human suggest that kynureninase activity may influence blood pressure and renal function. The gene coding kynureninase (KYNU) is also located on chromosome band 2q14-q23, where a linkage peak for essential hypertension was previously detected in the Chinese Han population.The results show that the rare KYNU variant Arg188Gln affects kynureninase activity and are consistent with the hypothesis that this mutation can predispose to essential hypertension.
References
Lima S, et al. (2007) Crystal structure of Homo sapiens kynureninase. Biochemistry. 46(10): 2735-44. Momany C, et al. (2004) Three-dimensional structure of kynureninase from Pseudomonas fluorescens. Biochemistry. 43(5): 1193-203. Toma S, et al. (1997) Cloning and recombinant expression of rat and human kynureninase. FEBS Lett. 408(1): 5-10.
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