Anti-PPM1A Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-PPM1A Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-PPM1A magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-PPM1A Magnetic Beads-IP Kit Product Description
The Anti-PPM1A magnetic Beads, conjugated with Anti-PPM1A antibody, are used for immuneprecipitation (IP) of PPM1A proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing PPM1A proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound PPM1A proteins are dissociated from the magnetic beads using an elution buffer. Anti-PPM1A Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Mouse PPM1A protein (Catalog#50277-M08E)
Species Reactivity
Mouse PPM1A / PP2C-alpha
Source
Polyclonal Mouse Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Mouse PPM1A (rM PPM1A; Catalog#50277-M08E; NP_032936.1; Met 1-Trp 382). PPM1A specific IgG was purified by mouse PPM1A affinity chromatography .
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-PPM1A Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-2310003C21RikALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-2900017D14RikALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-AI427932ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-AU017636ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-MMPa-2ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-MPPa-1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
PPM1A Background Information
Protein phosphatase 1A (PPM1A / PP2CA) is an enzyme belonging to the PP2C family of Ser / Thr protein phosphatases. Members of PP2C family are negative regulators of cell stress response pathways and the MAP kinases and MAP kinase kinases. It has also been demonstrated to inhibit the activation of p38 and JNK kinase cascades. PPM1A dephosphorylates and promotes nuclear export of TGFβ-activated Smad2/3. Ectopic expression of PPM1A abolishes TGFβ-induced antiproliferative and transcriptional responses, whereas depletion of PPM1A enhances TGFβ signaling in mammalian cells. It has been demonstrated that PPM1A / PP2CA, through dephosphorylation of Smad2/3, plays a critical role in terminating TGFβ signaling. Overexpression of PPM1A is reported to activate the expression of the tumor suppressor gene TP53 / p53, which leads to cell apoptosis.
Full Name
protein phosphatase, Mg2+/Mn2+ dependent, 1A
References
Lin X, et al. (2006) PPM1A functions as a Smad phosphatase to terminate TGFbeta signaling. Cell. 125(5): 915-28. Marc F, et al. (2003) Protein phosphatase 2C binds selectively to and dephosphorylates metabotropic glutamate receptor 3. Proc Natl Acad. 100 (26): 16006-11. Mann DJ, et al. (1992) Mammalian protein serine/threonine phosphatase 2C: cDNA cloning and comparative analysis of amino acid sequences. Biochim Biophys Acta. 1130 (1): 100-4.
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