Anti-CNDP2 Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-CNDP2 Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-CNDP2 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-CNDP2 Magnetic Beads-IP Kit Product Description
The Anti-CNDP2 magnetic Beads, conjugated with Anti-CNDP2 antibody, are used for immuneprecipitation (IP) of CNDP2 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing CNDP2 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound CNDP2 proteins are dissociated from the magnetic beads using an elution buffer. Anti-CNDP2 Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Mouse CNDP2 protein (Catalog#50246-M08B)
Species Reactivity
Mouse CNDP2
Source
Polyclonal Mouse Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Mouse CNDP2 (rM CNDP2; Catalog#50246-M08B; NP_075638.2; Met 1-Asn 475). CNDP2 specific IgG was purified by Mouse CNDP2 affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-CNDP2 Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-0610010E05RikALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-C76600ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-Cn2ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-Dip-2ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-Pep-1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-Pep1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
CNDP2 Background Information
Cytosolic non-specific dipeptidase, also known as CNDP dipeptidase 2, Glutamate carboxypeptidase-like protein 1, Peptidase A, CNDP2 and CN2, is a cytoplasm protein which belongs to the peptidase M2A family. CNDP2 / CPGL is a cytosolic enzyme that can hydrolyze carnosine to yield l-histidine and beta-alanine. CNDP2 / CPGL hydrolyzes a variety of dipeptides including L-carnosine but has a strong preference for Cys-Gly. It may be play a role as tumor suppressor in hepatocellular carcinoma (HCC) cells. Isoform 1 of CNDP2 / CPGL is ubiquitously expressed with higher levels in kidney and liver (at protein level). Isoform 2 of CNDP2 / CPGL is expressed in fetal tissues, it is only expressed in adult liver and placental tissues. CNDP2 / CPGL is highly expressed in the histaminergic neurons in the tuberomammillary nucleus, implying that it may supply histidine to histaminergic neurons for histamine synthesis.
Full Name
CNDP dipeptidase 2 (metallopeptidase M20 family)
References
Bakker,SJ. et al., 2008, Diabetes 57 (12):e16; author reply e17. Wanic, K. et al., 2008, Diabetes 57 (9): 2547-51. McDonough,CW. et al., 2009, Hum Genet 126 (2): 265-75. Kaur,H. et al., 2009, J Biol Chem. 284 (21):14493-502.
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