Anti-SerpinB8 Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-SerpinB8 Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-SerpinB8 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-SerpinB8 Magnetic Beads-IP Kit Product Description
The Anti-SerpinB8 magnetic Beads, conjugated with Anti-SerpinB8 antibody, are used for immuneprecipitation (IP) of SerpinB8 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing SerpinB8 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound SerpinB8 proteins are dissociated from the magnetic beads using an elution buffer. Anti-SerpinB8 Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Mouse SerpinB8 protein (Catalog#50215-M08B)
Species Reactivity
Mouse SERPINB8 / SerpinB8
Source
Polyclonal Mouse Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Mouse SerpinB8 (rM SerpinB8; Catalog#50215-M08B; NP_035589.1; Met 1-Pro 374). SerpinB8 specific IgG was purified by Mouse SerpinB8 affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-SerpinB8 Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-CAP-2ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-CAP2ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-NK10ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-ovalbuminALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-Spi8ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
SerpinB8 Background Information
Serpins are the largest and most diverse family of serine protease inhibitors which are involved in a number of fundamental biological processes such as blood coagulation, complement activation, fibrinolysis, angiogenesis, inflammation and tumor suppression and are expressed in a cell-specific manner.
Mouse SerpinB8, also known as Cytoplasmic antiproteinase 2, Peptidase inhibitor 8, SerpinB8, PI-8, SERPINB8 and CAP2, is a member of the Serpin superfamily. SERPINB8 was broadly expressed. In normal neuroendocrine tissues, strongest SerpinB8 expression was detected in islets of Langerhans of the pancreas. Moderate SerpinB8 expression was observed in neuroendocrine cells of the thyroid, adrenal cortex, colon, and pituitary gland. In the pancreas, SerpinB8 is specifically expressed by insulin-producing beta cells, and can be used as an additional diagnostic immunohistochemical marker. Mouse SerpinB8 distribution alters during kidney regeneration, possibly to control a prohormone convertase involved in inflammation or tissue repair.
Full Name
serpin peptidase inhibitor, clade B (ovalbumin), member 8
References
Sumi, Y. et al., 1989, J. Biochem. 106: 703-7. Rawlings, N.D. et al., 2004, Biochem J. 378: 705-16. Gillard, A. et al., 2006, Am J Nephrol. 26 (1): 34-42. Filleur, S. et al., 2009, J Cell Biochem. 106 (5): 769-75. de Koning, P.J. et al., 2009, Pancreas. 38 (4): 461-7.
新浪微博
腾讯微博
QQ空间
客服
