Anti-FGFRL1 Magnetic Beads-IP Kit Product Components
Components | Storage |
Anti-FGFRL1 Magnetic Beads1,3 | 2-8℃ for 12 months |
NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
5×TBST(pH7.4) | |
1×TBST(pH7.4) | |
ddH2O | |
CD166 Positive Cell Lysate | -20℃ for 12 months |
Alkaline Elution Buffer | 2-8℃ for 12 months |
Acidity Elution Buffer | 2-8℃ for 12 months |
Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-FGFRL1 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-FGFRL1 Magnetic Beads-IP Kit Product Description
The Anti-FGFRL1 magnetic Beads, conjugated with Anti-FGFRL1 antibody, are used for immuneprecipitation (IP) of FGFRL1 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing FGFRL1 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound FGFRL1 proteins are dissociated from the magnetic beads using an elution buffer. Anti-FGFRL1 Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Mouse FGFRL1 / FGFR5 protein (Catalog#50182-M08H)
Species Reactivity
Mouse FGFRL1 / FGFR5
Source
Monoclonal Mouse Rabbit IgG
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Mouse FGFRL1 / FGFR5 (rM FGFRL1 / FGFR5; Catalog#50182-M08H; NP_473412.1; Met1-Pro374).
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-FGFRL1 Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-FGFR5ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-FGFR5betaALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-FGFR5gammaALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
FGFRL1 Background Information
Fibroblast growth factor receptor-like 1 (FGFRL1) also known as Fibroblast growth factor receptor 5 (FGFR5), is a member of the fibroblast growth factor receptor (FGFR) family, where amino acid sequence is highly conserved between members and throughout evolution. A full-length representative protein would consist of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. A unique feature of FGFRL1/FGFR5 is that it does not contain an intracellular tyrosine kinase domain. Some muscle types, including the muscles of the tongue and the diaphragm, express FGFRL1/FGFR5 at relatively high level. In contrast, the heart and the skeletal muscles of the limbs, as well as many other organs (brain, lung, liver, kidney, gut) express Fgfrl1 only at basal level. It is conceivable that FGFRL1/FGFR5 interacts with other Fgfrs, which are expressed in cartilage and muscle, to modulate FGF signaling.
Immune Checkpoint Immunotherapy Cancer Immunotherapy Targeted Therapy Full Name
fibroblast growth factor receptor-like 1
References
Wiedemann M, et al. (2000) Characterization of a novel protein (FGFRL1) from human cartilage related to FGF receptors. Genomics. 69(2): 275-9. Trueb B, et al. (2006) Expression of FGFRL1, a novel fibroblast growth factor receptor, during embryonic development. Int J Mol Med. 17(4): 617-20. Wiedemann M, et al. (2001) The mouse Fgfrl1 gene coding for a novel FGF receptor-like protein. Biochim Biophys Acta. 1520(3): 247-50. Trueb B, et al. (2003) Characterization of FGFRL1, a novel fibroblast growth factor (FGF) receptor preferentially expressed in skeletal tissues. J Biol Chem. 278(36): 33857-65.