Anti-FGF18 Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-FGF18 Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-FGF18 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-FGF18 Magnetic Beads-IP Kit Product Description
The Anti-FGF18 magnetic Beads, conjugated with Anti-FGF18 antibody, are used for immuneprecipitation (IP) of FGF18 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing FGF18 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound FGF18 proteins are dissociated from the magnetic beads using an elution buffer. Anti-FGF18 Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Mouse FGF18 protein (Catalog#50177-M08H)
Species Reactivity
Mouse FGF18
Source
Polyclonal Mouse Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Mouse FGF18 (rh FGF18; Catalog#50177-M08H; NP_032031.1; Met1-Gly207). FGF18 specific IgG was purified by Mouse FGF18 affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-FGF18 Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-D130055P09RikALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-FGF-18ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
FGF18 Background Information
Fibroblast growth factor 18 (FGF18) is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth, and invasion. It has been shown in vitro that FGF18 is able to induce neurite outgrowth in PC12 cells. Studies of the similar proteins in mouse and chick suggested that this protein is a pleiotropic growth factor that stimulates proliferation in a number of tissues, most notably the liver and small intestine. Experiment datas identified FGF18 as a selective ligand for FGFR3 in limb bud mesenchymal cells, which suppressed proliferation and promoted their differentiation and production of cartilage matrix. FGF18 appears to regulate cell proliferation and differentiation positively in osteogenesis and negatively in chondrogenesis.
Full Name
fibroblast growth factor 18
References
Ohbayashi N, et al. (2002) FGF18 is required for normal cell proliferation and differentiation during osteogenesis and chondrogenesis. Genes Dev. 16(7): 870-9. Davidson D, et al. (2005) Fibroblast growth factor (FGF) 18 signals through FGF receptor 3 to promote chondrogenesis. J Biol Chem. 280(21): 20509-15. Liu Z, et al. (2007) FGF18 is required for early chondrocyte proliferation, hypertrophy and vascular invasion of the growth plate. Dev Biol. 302(1): 80-91.
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