Anti-Asialoglycoprotein Receptor 1 Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-Asialoglycoprotein Receptor 1 Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-Asialoglycoprotein Receptor 1 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-Asialoglycoprotein Receptor 1 Magnetic Beads-IP Kit Product Description
The Anti-Asialoglycoprotein Receptor 1 magnetic Beads, conjugated with Anti-Asialoglycoprotein Receptor 1 antibody, are used for immuneprecipitation (IP) of Asialoglycoprotein Receptor 1 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing Asialoglycoprotein Receptor 1 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound Asialoglycoprotein Receptor 1 proteins are dissociated from the magnetic beads using an elution buffer. Anti-Asialoglycoprotein Receptor 1 Magnetic Beads-IP Kit Antibody Information
Antibody
Anti-Asialoglycoprotein Receptor 1 Antibody(
50083-RP02)
Immunogen
Recombinant Mouse ASGPR1 / ASGR1 protein (Catalog#50083-M07H)
Species Reactivity
Mouse ASGPR1 / ASGR1
Source
Polyclonal Mouse Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Mouse ASGPR1 / ASGR1 (rM ASGPR1 / ASGR1; Catalog#50083-M07H; NP_033844.1; Ser 60-Asn 284). ASGPR1 / ASGR1 specific IgG was purified by Mouse ASGPR1 / ASGR1 affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-Asialoglycoprotein Receptor 1 Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-ASGPR1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-AsgrALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-Asgr-1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-HL-1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
Asialoglycoprotein Receptor 1 Background Information
The asialoglycoprotein receptor (ASGPR), an endocytotic cell surface receptor expressed by hepatocytes, is triggered by triantennary binding to galactose residues of macromolecules such as asialoorosomucoid (ASOR). ASGPR belongs to the long-form subfamily of the C-type/Ca2+ dependent lectin family. It is a complex of two noncovalently-linked and highly homologous subunits, a major 42 kDa glycoprotein ASGPR1(MHL-1) and a minor 51 kDa glycoprotein ASGR2 (MHL-2). ASGPR1 is synthesized as a type II transmembrane protein that contains a cytosolic N-terminal domain, a single transmembrane segment, and an extracellular domain which contains two important structural regions. The first is a stalk domain that contributes to noncovalent oligomerization, and the second is a Ca2+-dependent carbohydrate binding domain at the very C-terminus that is unusually stabilized by three ions. The research regarded that ASGPR1 could be targeted for anti- hepatitis B virus (HBV) drug development.
Full Name
asialoglycoprotein receptor 1
References
Yang J, et al. (2006) Antisense oligonucleotides targeted against asialoglycoprotein receptor 1 block human hepatitis B virus replication. J Viral Hepat. 13(3): 158-65. Li Y, et al. (2008) Targeted delivery of macromolecular drugs: asialoglycoprotein receptor (ASGPR) expression by selected hepatoma cell lines used in antiviral drug development. Curr Drug Deliv. 5(4): 299-302.
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