Anti-CD86 Magnetic Beads Immunoprecipitation (IP) Kit 5mL

Anti-CD86 Magnetic Beads-IP Kit Product Components

Components	Storage
Anti-CD86 Magnetic Beads^1,3	2-8℃ for 12 months
NP40 Cell Lysis Buffer²	-20℃ for 12 months
5×TBST（pH7.4）
1×TBST（pH7.4）
ddH₂O
CD166 Positive Cell Lysate	-20℃ for 12 months
Alkaline Elution Buffer	2-8℃ for 12 months
Acidity Elution Buffer	2-8℃ for 12 months
Neutralization Buffer	2-8℃ for 12 months

Components

Storage

Anti-CD86 Magnetic Beads^1,3

2-8℃ for 12 months

NP40 Cell Lysis Buffer²

-20℃ for 12 months

5×TBST（pH7.4）

1×TBST（pH7.4）

ddH₂O

CD166 Positive Cell Lysate

-20℃ for 12 months

Alkaline Elution Buffer

2-8℃ for 12 months

Acidity Elution Buffer

2-8℃ for 12 months

Neutralization Buffer

2-8℃ for 12 months

[1] The IP KIT contains anti-CD86 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).

[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.

[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.

Anti-CD86 Magnetic Beads-IP Kit Product Description

The Anti-CD86 magnetic Beads, conjugated with Anti-CD86 antibody, are used for immuneprecipitation (IP) of CD86 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing CD86 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound CD86 proteins are dissociated from the magnetic beads using an elution buffer.

Anti-CD86 Magnetic Beads-IP Kit Antibody Information

Antibody

Anti-CD86 Antibody( 50068-RP02)

Immunogen

Recombinant Mouse CD86 protein (Catalog#50068-M08H)

Species Reactivity

Mouse CD86 / ETC-1 / B7-2

Source

Polyclonal Mouse Rabbit IgG

Preparation

Produced in rabbits immunized with purified, recombinant Mouse CD86 extracellular domain (rM CD86; Catalog#50068-M08H; NP_062261.3; Met 1-Glu 245). CD86 specific IgG was purified by mouse CD86 affinity chromatography.

Applications

Immunoprecipitation (IP), Minimum Protein Purification

Anti-CD86 Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names

Anti-B7ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-B7-2ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-B7.2ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-B70ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-Cd28l2ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-CLS1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-ETC-1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-Ly-58ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-Ly58ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-MB7ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-MB7-2ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-TS/A-2ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit

CD86 Background Information

CD86, also known as B-lymphocyte activation antigen B7-2 (referred to as B7), is a member of the cell surface immunoglobulin superfamily. B7-2 exists predominantly as a monomer on cell surfaces and interacts with two co-stimulatory receptors CD28 and cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) expressed on T cells, and thus induces the signal pathways which regulate T cell activation and tolerance, cytokine production, and the generation of CTL. It is indicated that contacts between B and T helper cells mediated by CD86 encourage signals for the proliferation and IgG secretion of normal B cells and B cell lymphomas. Recent study has revealed that CD86 also promotes the generation of a mature APC repertoire and promotes APC function and survival. CD86 has an important role in chronic hemodialysis, allergic pulmonary inflammation, arthritis, and antiviral responses, and thus is regarded as a promising candidate for immune therapy. Immune Checkpoint
Immune Checkpoint Detection: Antibodies Immune Checkpoint Detection: ELISA Antibodies Immune Checkpoint Detection: IP Antibodies Immune Checkpoint Detection: FCM Antibodies Immune Checkpoint Detection: WB Antibodies
Immune Checkpoint Proteins
Immune Checkpoint Targets Co-inhibitory Immune Checkpoint Targets Immunotherapy Cancer Immunotherapy Targeted Therapy

Full Name

CD86 antigen

References

Chen YQ, et al. (2006) CD28/CTLA-4--CD80/CD86 and ICOS--B7RP-1 costimulatory pathway in bronchial asthma. Allergy. 61(1): 15-26.

Rau FC, et al. (2009) B7-1/2 (CD80/CD86) direct signaling to B cells enhances IgG secretion. J Immunol. 183(12): 7661-71.

Dai ZS, et al. (2009) Defective expression and modulation of B7-2/CD86 on B cells in B cell chronic lymphocytic leukemia. Int J Hematol. 89(5): 656-63.

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品