Anti-CD86 Magnetic Beads-IP Kit Product Components
Components | Storage |
Anti-CD86 Magnetic Beads1,3 | 2-8℃ for 12 months |
NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
5×TBST(pH7.4) | |
1×TBST(pH7.4) | |
ddH2O | |
CD166 Positive Cell Lysate | -20℃ for 12 months |
Alkaline Elution Buffer | 2-8℃ for 12 months |
Acidity Elution Buffer | 2-8℃ for 12 months |
Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-CD86 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-CD86 Magnetic Beads-IP Kit Product Description
The Anti-CD86 magnetic Beads, conjugated with Anti-CD86 antibody, are used for immuneprecipitation (IP) of CD86 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing CD86 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound CD86 proteins are dissociated from the magnetic beads using an elution buffer. Anti-CD86 Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Mouse CD86 protein (Catalog#50068-M08H)
Species Reactivity
Mouse CD86 / ETC-1 / B7-2
Source
Polyclonal Mouse Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Mouse CD86 extracellular domain (rM CD86; Catalog#50068-M08H; NP_062261.3; Met 1-Glu 245). CD86 specific IgG was purified by mouse CD86 affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-CD86 Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-B7ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-B7-2ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-B7.2ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-B70ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-Cd28l2ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-CLS1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-ETC-1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-Ly-58ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-Ly58ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-MB7ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-MB7-2ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-TS/A-2ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
CD86 Background Information
References
Chen YQ, et al. (2006) CD28/CTLA-4--CD80/CD86 and ICOS--B7RP-1 costimulatory pathway in bronchial asthma. Allergy. 61(1): 15-26. Rau FC, et al. (2009) B7-1/2 (CD80/CD86) direct signaling to B cells enhances IgG secretion. J Immunol. 183(12): 7661-71. Dai ZS, et al. (2009) Defective expression and modulation of B7-2/CD86 on B cells in B cell chronic lymphocytic leukemia. Int J Hematol. 89(5): 656-63.