Anti-MIF Magnetic Beads-IP Kit Product Components
Components | Storage |
Anti-MIF Magnetic Beads1,3 | 2-8℃ for 12 months |
NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
5×TBST(pH7.4) | |
1×TBST(pH7.4) | |
ddH2O | |
CD166 Positive Cell Lysate | -20℃ for 12 months |
Alkaline Elution Buffer | 2-8℃ for 12 months |
Acidity Elution Buffer | 2-8℃ for 12 months |
Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-MIF magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-MIF Magnetic Beads-IP Kit Product Description
The Anti-MIF magnetic Beads, conjugated with Anti-MIF antibody, are used for immuneprecipitation (IP) of MIF proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing MIF proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound MIF proteins are dissociated from the magnetic beads using an elution buffer. Anti-MIF Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Mouse MIF protein (Catalog#50066-M08H)
Species Reactivity
Mouse MIF
Source
Polyclonal Mouse Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Mouse MIF (rh MIF; Catalog#50066-M08H; P34884; Pro2-Ala115). MIF specific IgG was purified by Mouse MIF affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-MIF Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-GIFALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-GlifALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
MIF Background Information
Macrophage migration inhibitory factor (MIF) is an immunoregulatory cytokine, the effect of which on arresting random immune cell movement was recognized several decades ago. Despite its historic name, MIF also has a direct chemokine-like function and promotes cell recruitment. MIF is an ubiquitously expressed protein that plays a crucial role in many inflammatory and autoimmune disorders. Increasing evidence suggests that MIF also controls metabolic and inflammatory processes underlying the development of metabolic pathologies associated with obesity. Further research has shown that MIF plays a particularly critical part in cell cycle regulation and therefore in tumorigenesis as well. The significance of the role of MIF in a variety of both solid and hematologic tumors has been established. More recently, interest has increased in the role of MIF in the development of central nervous system (CNS) tumors, in which it appears to influence cell cycle control. MIF contributes to malignant disease progression on several different levels. Both circulating and intracellular MIF protein levels are elevated in cancer patients and MIF expression reportedly correlates with stage, metastatic spread and disease-free survival. Blockade of MIF bioactivity successfully inhibited tumor cell growth in vivo and in vitro. MIF plays important roles in the pathogenesis of gastrointestinal, hepatic, and pancreatic disorders.
Full Name
macrophage migration inhibitory factor (glycosylation-inhibiting factor)
References
Ohkawara T, et al. (2005) Pathophysiological roles of macrophage migration inhibitory factor in gastrointestinal, hepatic, and pancreatic disorders. J Gastroenterol. 40(2): 117-22. Bach JP, et al.. (2009) The role of macrophage inhibitory factor in tumorigenesis and central nervous system tumors. Cancer. 115(10): 2031-40. Rendon BE, et al.. (2009) Mechanisms of macrophage migration inhibitory factor (MIF)-dependent tumor microenvironmental adaptation. Exp Mol Pathol. 86(3): 180-5. Grieb G, et al. (2010) Macrophage migration inhibitory factor (MIF): a promising biomarker. Drug News Perspect. 23(4): 257-64.