Anti-ARSA Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-ARSA Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-ARSA magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-ARSA Magnetic Beads-IP Kit Product Description
The Anti-ARSA magnetic Beads, conjugated with Anti-ARSA antibody, are used for immuneprecipitation (IP) of ARSA proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing ARSA proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound ARSA proteins are dissociated from the magnetic beads using an elution buffer. Anti-ARSA Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Mouse ARSA protein (Catalog#50018-M08H)
Species Reactivity
Mouse Arylsulfatase-A / ARSA
Source
Polyclonal Mouse Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Mouse ARSA (rM ARSA; Catalog#50018-M08H; NP_033843.2; Met 1-Ser 506). ARSA specific IgG was purified by Mouse ARSA affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-ARSA Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-As-2ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-AS-AALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-As2ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-ASAALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-AW212749ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-TISP73ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
ARSA Background Information
Arylsulfatase A (ARSA) is synthesized as a 52KDa lysosomal enzyme. It is a member of the sulfatase family that is required for the lysosomal degradation of cerebroside-3-sulfate, a sphingolipid sulfate ester and a major constituent of the myelin sheet. Arylsulfatase A is activated by a required co- or posttranslational modification with the oxidation of cysteine to formylglycine. Metachromatic leukodystrophy (MLD) is a lysosomal storage disease in the central and peripheral nervous systems with severe and progressive neurological symptoms caused by the deficiency of Arylsulfatase A. Deficiency of this enzyme is also found in apparently healthy individuals, a condition for which the term pseudodeficiency is introduced. ARSA forms dimers after receiving three N-linked oligosaccharides in the endoplasmic reticulum, and then the dimers are transported to the Golgi where they receive mannose 6-phosphate recognition markers. And thus, ARSA is transported and delivered to dense lysosomes in a mannose 6-phosphate receptor-dependent manner. It has been shown that within the lysosomes, the ARSA dimers can oligomerize to an octamer in a pH-dependent manner. The ARSA deficiency leads to metachromatic leucodystrophy (MLD), a lysosomal storage disorder associated with severe and progressive demyelination in he central and peripheral nervous system. Additionally, the serum level of arylsulfatase A might be helpful in diagnosis of lung and central nervous system cancer.
Full Name
arylsulfatase A
References
Laidler PM. (1991) Arylsulfatase A--physico-chemical properties and the use of enzyme radioimmunoassay in medical diagnosis Folia Med Cracov. 32(3-4): 149-68. Jean S, et al. (2006) Ethanol decreases rat hepatic arylsulfatase A activity levels. Alcohol Clin Exp Res. 30(11): 1950-5.
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