Anti-HIV gp120 Magnetic Beads-IP Kit Product Components
Components | Storage |
Anti-HIV gp120 Magnetic Beads1,3 | 2-8℃ for 12 months |
NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
5×TBST(pH7.4) | |
1×TBST(pH7.4) | |
ddH2O | |
CD166 Positive Cell Lysate | -20℃ for 12 months |
Alkaline Elution Buffer | 2-8℃ for 12 months |
Acidity Elution Buffer | 2-8℃ for 12 months |
Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-HIV gp120 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-HIV gp120 Magnetic Beads-IP Kit Product Description
The Anti-HIV gp120 magnetic Beads, conjugated with Anti-HIV gp120 antibody, are used for immuneprecipitation (IP) of HIV gp120 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing HIV gp120 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound HIV gp120 proteins are dissociated from the magnetic beads using an elution buffer. Anti-HIV gp120 Magnetic Beads-IP Kit Antibody Information
Antibody
HIV-1(group P, strain RBF168) gp120 Antibody, Rabbit PAb, Antigen Affinity Purified(
40259-T60)
Immunogen
Recombinant HIV-1(group P, strain RBF168) gp120 protein (Catalog#40259-V08H1)
Species Reactivity
HIV-1(group P, strain RBF168)
Source
Polyclonal HIV Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant HIV-1(group P, strain RBF168) gp120 (rh HIV-1(group P, strain RBF168) gp120 ; Catalog#40259-V08H1; ACY40659.1; Tyr38-Arg527). HIV-1(group P, strain RBF168) gp120 specific IgG was purified by HIV-1(group P, strain RBF168) gp120 affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
HIV gp120 Background Information
The HIV-1 gp12 envelope protein, a glycoprotein that is part of the outer layer of the virus, which is an essential component in the multi-tiered viral entry process. It presents itself as viral membrane spikes consisting of 3 molecules of gp12 linked together and anchored to the membrane by gp41 protein. Gp12 is essential for viral infection as it facilitates HIV entry into the host cell and this is its best-known and most researched role in HIV infection. However, it is becoming increasingly evident that gp12 might also be facilitating viral persistence and continuing HIV infection by influencing the T cell immune response to the virus. The surface protein gp12 attaches the virus to the host lymphoid cell by binding to the primary receptor CD4. Gp12 binding to its receptor CD4 and co-receptor, CXCR4 or CCR5 is required for fusion of viral and cellular membranes. Several mechanisms might be involved in this process of which gp12 binding to the CD4 receptor of T cells is the best known and most important interaction as it facilitates viral entry into the CD4+ cells and their depletion, a hallmark of the HIV infection. Gp12 is shed from the viral membrane and accumulates in lymphoid tissues in significant amounts. Despite the overall genetic heterogeneity of the gp12 glycoprotein, the conserved CD4 binding site provides an attractive antiviral target. Interaction between gp12 and ITGA4/ITGB7 would allow the virus to enter GALT early in the infection, infecting and killing most of GALT's resting CD4+ T-cells. This T-cell depletion is believed to be the major insult to the host immune system leading to AIDS.
References
Kadow J, et al. (2006) Small-molecule HIV-1 gp120 inhibitors to prevent HIV-1 entry: an emerging opportunity for drug development. Curr Opin Investig Drugs. 7(8): 721-6. Stevceva L, et al. (2007) Immune responses to HIV Gp120 that facilitate viral escape. Curr HIV Res. 5(1): 47-54. Yoon V, et al. (2010) The GP120 molecule of HIV-1 and its interaction with T cells. Curr Med Chem. 17(8): 741-9.