Anti-CoV spike Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-CoV spike Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-CoV spike magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-CoV spike Magnetic Beads-IP Kit Product Description
The Anti-CoV spike magnetic Beads, conjugated with Anti-CoV spike antibody, are used for immuneprecipitation (IP) of CoV spike proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing CoV spike proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound CoV spike proteins are dissociated from the magnetic beads using an elution buffer. Anti-CoV spike Magnetic Beads-IP Kit Antibody Information
Antibody
MERS-CoV (NCoV / Novel coronavirus) Spike Protein S2 Antibody, Rabbit PAb, Antigen Affinity Purified(
40070-T60)
Immunogen
Recombinant MERS-CoV (NCoV / Novel coronavirus) Spike glycoprotein Protein (Catalog#40070-V08B)
Species Reactivity
MERS-CoV (NCoV / Novel coronav
Source
Polyclonal MERS-CoV Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant MERS-CoV (NCoV / Novel coronavirus) Spike Protein S2 (Catalog#40070-V08B; AFS88936.1; Asp726-Pro1296), and purified by antigen affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Immunoprecipitation/ IP Kit- Anti-CoV Spike glycoprotein Immunomagnetic Beads Alternative Names
Anti-SALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
CoV spike Background Information
The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell: they are essential for both host specificity and viral infectivity. The term 'peplomer' is typically used to refer to a grouping of heterologous proteins on the virus surface that function together. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. Most notable is severe acute respiratory syndrome (SARS). The severe acute respiratory syndrome-coronavirus (SARS-CoV) spike (S) glycoprotein alone can mediate the membrane fusion required for virus entry and cell fusion. It is also a major immunogen and a target for entry inhibitors. The SARS-CoV spike (S) protein is composed of two subunits; the S1 subunit contains a receptor-binding domain that engages with the host cell receptor angiotensin-converting enzyme 2 and the S2 subunit mediates fusion between the viral and host cell membranes. The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity, during infection with SARS-CoV.
References
Shen S, et al. (2007) Expression, glycosylation, and modification of the spike (S) glycoprotein of SARS CoV. Methods Mol Biol. 379: 127-35. Du L, et al. (2009) The spike protein of SARS-CoV--a target for vaccine and therapeutic development. Nat Rev Microbiol. 7 (3): 226-36. Xiao X, et al. (2004) The SARS-CoV S glycoprotein. Cell Mol Life Sci. 61 (19-20): 2428-30.
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