Anti-CoV Nucleoprotein Magnetic Beads-IP Kit Product Components
Components | Storage |
Anti-CoV Nucleoprotein Magnetic Beads1,3 | 2-8℃ for 12 months |
NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
5×TBST(pH7.4) | |
1×TBST(pH7.4) | |
ddH2O | |
CD166 Positive Cell Lysate | -20℃ for 12 months |
Alkaline Elution Buffer | 2-8℃ for 12 months |
Acidity Elution Buffer | 2-8℃ for 12 months |
Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-CoV Nucleoprotein magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-CoV Nucleoprotein Magnetic Beads-IP Kit Product Description
The Anti-CoV Nucleoprotein magnetic Beads, conjugated with Anti-CoV Nucleoprotein antibody, are used for immuneprecipitation (IP) of CoV Nucleoprotein proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing CoV Nucleoprotein proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound CoV Nucleoprotein proteins are dissociated from the magnetic beads using an elution buffer. Anti-CoV Nucleoprotein Magnetic Beads-IP Kit Antibody Information
Antibody
MERS-CoV (NCoV / Novel coronavirus) Nucleocapsid Antibody, Mouse MAb(
40068-MM10)
Immunogen
Recombinant MERS-CoV (NCoV / Novel coronavirus) Nucleoprotein / NP protein (Catalog#40068-V08B)
Species Reactivity
The antibody reacts with MERS-
Source
Monoclonal MERS-CoV Mouse IgG1
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant MERS-CoV (NCoV / Novel coronavirus) Nucleoprotein / NP (Catalog#40068-V08B; AFS88943.1; Met 1-Asp413). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Immunoprecipitation/ IP Kit- Anti-CoV Nucleoprotein Immunomagnetic Beads Alternative Names
Anti-NPALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
CoV Nucleoprotein Background Information
Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronaviruses primarily cause respiratory and enteric diseases in mammals and birds. Coronaviruses can cause a range of symptoms varying from mild symptoms such as the common cold to more serious respiratory illnesses. They primarily cause respiratory and enteric diseases in mammals and birds. Coronavirus symptoms include rhinorrhea, sneezing, cough, nasal obstruction, bronchitis and so on. There are three main groups of coronaviruses: alpha, beta, and gamma. Proteins that contribute to the overall structure of all coronaviruses are the spike (S), envelope (E), membrane (M) and nucleoprotein (N). Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch.
References
- 1.Van Boheemen S, et al. (2012), MBio. 3(6):e00473-12.
- Bisht H. et al., 2004, Proc Natl Acad Sci. 101 (17): 6641-6.
- Li W. et al., 2005, Science. 309 (5742): 1864-8.