Anti-Matrix protein 1 / M1 Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-Matrix protein 1 / M1 Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-Matrix protein 1 / M1 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-Matrix protein 1 / M1 Magnetic Beads-IP Kit Product Description
The Anti-Matrix protein 1 / M1 magnetic Beads, conjugated with Anti-Matrix protein 1 / M1 antibody, are used for immuneprecipitation (IP) of Matrix protein 1 / M1 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing Matrix protein 1 / M1 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound Matrix protein 1 / M1 proteins are dissociated from the magnetic beads using an elution buffer. Anti-Matrix protein 1 / M1 Magnetic Beads-IP Kit Antibody Information
Antibody
Influenza A H1N1 (A/Puerto Rico/8/34/Mount Sinai) Matrix protein 1 / M1 Antibody, Rabbit PAb, Antigen Affinity Purified(
40010-T60)
Immunogen
Recombinant Influenza A H1N1 (A/Puerto Rico/8/34/Mount Sinai) Matrix protein 1 / M1 Protein (Catalog#40010-V07E)
Species Reactivity
Influenza A H1N1 (A/Puerto Ric
Source
Polyclonal H1N1 Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Influenza A H1N1 (A/Puerto Rico/8/34/Mount Sinai) Matrix protein 1 / M1 (Catalog#40010-V07E; NP_040978.1; Ser2-Lys252). Influenza A H1N1 (A/Puerto Rico/8/34/Mount Sinai) Matrix protein 1 / M1 specific IgG was purified by Influenza A H1N1 (A/Puerto Rico/8/34/Mount Sinai) Matrix protein 1 / M1 affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Matrix protein 1 / M1 Background Information
Influenza virus matrix protein 1 (M1) has been shown to play a crucial role in the virus replication, assembly and budding.The matrix protein 1 (M1) is the most abundant structural protein in influenza A virus particles.Matrix protein 1 (M1) of the influenza A virus plays multiple roles in virion assembly and infection. M1 protein was a candidate antigen for a broad-spectrum influenza virus vaccine and the adjuvant chitosan significantly improved the efficacy of the M1 vaccine.
Full Name
cholinergic receptor, muscarinic 1
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