Anti-EDEM2 Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-EDEM2 Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-EDEM2 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-EDEM2 Magnetic Beads-IP Kit Product Description
The Anti-EDEM2 magnetic Beads, conjugated with Anti-EDEM2 antibody, are used for immuneprecipitation (IP) of EDEM2 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing EDEM2 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound EDEM2 proteins are dissociated from the magnetic beads using an elution buffer. Anti-EDEM2 Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Human EDEM2 / C20orf31 Protein (Catalog#15687-H08H)
Species Reactivity
Human EDEM2 / C20orf31
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Human EDEM2 / C20orf31 (rh EDEM2 / C20orf31; Catalog#15687-H08H; AAH01371.1; Met1-Lys492). EDEM2 / C20orf31 specific IgG was purified by Human EDEM2 / C20orf31 affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-EDEM2 Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-bA4204.1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-C20orf31ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-C20orf49ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-UNQ573/PRO1135ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
EDEM2 Background Information
EDEM2, also known as C2orf31, belongs to a family of proteins involved in ER-associated degradation (ERAD) of glycoproteins. In the endoplasmic reticulum (ER), misfolded proteins are retrotranslocated to the cytosol and degraded by the proteasome. Early in this pathway, a proposed lumenal ER lectin, EDEM, recognizes misfolded glycoproteins in the ER, disengages the nascent molecules from the folding pathway, and facilitates their targeting for disposal. In humans there are a total of three EDEM homologs. The amino acid sequences of these proteins are different from other lectins but are closely related to the Class I mannosidases (family 47 glycosidases). EDEM2 is one of the EDEM homologs. Overexpression of EDEM2 accelerates the degradation of misfolded alpha1-antitrypsin, indicating that the protein is involved in ERAD.
Full Name
ER degradation enhancer, mannosidase alpha-like 2
References
Mast SW. et al., 2005, Glycobiology. 15 (4): 421-36. Olivari S. et al., 2005, J Biol Chem. 280 (4): 2424-8. Ota T. et al., 2004, Nat Genet. 36 (1): 40-5.
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