Anti-p67phox Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-p67phox Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-p67phox magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-p67phox Magnetic Beads-IP Kit Product Description
The Anti-p67phox magnetic Beads, conjugated with Anti-p67phox antibody, are used for immuneprecipitation (IP) of p67phox proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing p67phox proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound p67phox proteins are dissociated from the magnetic beads using an elution buffer. Anti-p67phox Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Human NCF2 / NCF-2 / P67phox protein (Catalog#15669-H20B)
Species Reactivity
Human NCF2 / NCF-2 / P67phox
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Human NCF2 / NCF-2 / P67phox (rh NCF2 / NCF-2 / P67phox; Catalog#15669-H20B; AAH01606.1; Met1-Val526). NCF2 / NCF-2 / P67phox specific IgG was purified by Human NCF2 / NCF-2 / P67phox affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-p67phox Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-NCF-2ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-NOXA2ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-P67-PHOXALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-P67PHOXALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
p67phox Background Information
NCF2, also known as NCF-2 and p67phox, is a subunit of the multi-protein NADPH oxidase complex. NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase. This oxidase produces a burst of superoxide which is delivered to the lumen of the neutrophil phagosome. Mutations in NCF2 gene, as well as in other NADPH oxidase subunits, can result in chronic granulomatous disease, a disease that causes recurrent infections by catalase-positive organisms.
Full Name
neutrophil cytosolic factor 2
References
Wientjes FB. et al., 1996, Semin Cell Biol. 6 (6): 357-65. DeLeo FR. et al., 1997, J Leukoc Biol. 60 (6): 677-91. Dorseuil O. et al., 1997, C R Seances Soc Biol Fil. 191 (2): 237-46.
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