Anti-COX5B Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-COX5B Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-COX5B magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-COX5B Magnetic Beads-IP Kit Product Description
The Anti-COX5B magnetic Beads, conjugated with Anti-COX5B antibody, are used for immuneprecipitation (IP) of COX5B proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing COX5B proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound COX5B proteins are dissociated from the magnetic beads using an elution buffer. Anti-COX5B Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Human COX5B protein (Catalog#15660-H07E)
Species Reactivity
Human COX5B
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Human COX5B (rh COX5B; Catalog#15660-H07E; P10606; Ala32-His129). COX5B specific IgG was purified by Human COX5B affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-COX5B Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-COXVBALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
COX5B Background Information
Cytochrome C oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit Vb of the human mitochondrial respiratory chain enzyme.
Full Name
cytochrome c oxidase subunit Vb
References
Romero N. et al., 1990, Histochemistry. 94 (2): 211-5. Lomax MI. et al., 1991, Genomics. 10 (1): 1-9. Beauchemin A M. et al., 2001, Brain Res Bull. 56 (3-4): 285-97.
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