Anti-PIG3 Magnetic Beads-IP Kit Product Components
Components | Storage |
Anti-PIG3 Magnetic Beads1,3 | 2-8℃ for 12 months |
NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
5×TBST(pH7.4) | |
1×TBST(pH7.4) | |
ddH2O | |
CD166 Positive Cell Lysate | -20℃ for 12 months |
Alkaline Elution Buffer | 2-8℃ for 12 months |
Acidity Elution Buffer | 2-8℃ for 12 months |
Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-PIG3 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-PIG3 Magnetic Beads-IP Kit Product Description
The Anti-PIG3 magnetic Beads, conjugated with Anti-PIG3 antibody, are used for immuneprecipitation (IP) of PIG3 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing PIG3 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound PIG3 proteins are dissociated from the magnetic beads using an elution buffer. Anti-PIG3 Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Human PIG3 / TP53I3 protein (Catalog#15531-H07E)
Species Reactivity
Human PIG3 / TP53I3
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Human PIG3 / TP53I3 (rh PIG3 / TP53I3; Catalog#15531-H07E; NP_004872.2; Met1-Gln332). PIG3 / TP53I3 specific IgG was purified by Human PIG3 / TP53I3 affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-PIG3 Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-PIG3ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-TP53I3ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
PIG3 Background Information
Nutlin-3 variably induces apoptosis and cell cycle arrest in cancer cells while it shows low/absent cytotoxicity in normal cells, Nutlin-3 is a promising anti-cancer agent, which exhibits activity against a variety of cancers, including acute myeloid leukemia (AML). The important role of TP53I3/PIG3 in mediating the apoptotic activity of Nutlin-3 was underlined by knock-down experiments with siRNA specific for TP53I3/PIG3, which resulted in a significant decrease in the pro-apoptotic activity of Nutlin-3.
Full Name
tumor protein p53 inducible protein 3