Anti-MAGOH Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-MAGOH Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-MAGOH magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-MAGOH Magnetic Beads-IP Kit Product Description
The Anti-MAGOH magnetic Beads, conjugated with Anti-MAGOH antibody, are used for immuneprecipitation (IP) of MAGOH proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing MAGOH proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound MAGOH proteins are dissociated from the magnetic beads using an elution buffer. Anti-MAGOH Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Human MAGOH Protein (Catalog#15516-H08E)
Species Reactivity
Human MAGOH
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Human MAGOH (rh MAGOH; Catalog#15516-H08E; NP_002361.1; Met1-Ile146). MAGOH specific IgG was purified by Human MAGOH affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-MAGOH Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-MAGOH1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-MAGOHAALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
MAGOH Background Information
MAGOH is the human homologue of Drosophila mago nashi, a protein that is required for normal germ plasm development in the Drosophila embryo.EJCs are deposited on messenger RNAs (mRNAs) during splicing and their core consists of eIF4A3, MLN51, Y14, and MAGOH.Both MAGOH proteins interact with other EJC components, incorporate into mRNA-bound EJCs, and activate nonsense-mediated decay.
Full Name
mago-nashi homolog, proliferation-associated (Drosophila)
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