Anti-Glycerol 3 Phosphate Dehydrogenase Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-Glycerol 3 Phosphate Dehydrogenase Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-Glycerol 3 Phosphate Dehydrogenase magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-Glycerol 3 Phosphate Dehydrogenase Magnetic Beads-IP Kit Product Description
The Anti-Glycerol 3 Phosphate Dehydrogenase magnetic Beads, conjugated with Anti-Glycerol 3 Phosphate Dehydrogenase antibody, are used for immuneprecipitation (IP) of Glycerol 3 Phosphate Dehydrogenase proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing Glycerol 3 Phosphate Dehydrogenase proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound Glycerol 3 Phosphate Dehydrogenase proteins are dissociated from the magnetic beads using an elution buffer. Anti-Glycerol 3 Phosphate Dehydrogenase Magnetic Beads-IP Kit Antibody Information
Antibody
Anti-Glycerol 3 Phosphate Dehydrogenase Antibody(
15489-T36)
Immunogen
Recombinant Human GPD1 / Glycerolphosphate Dehydrogenase 1 protein (Catalog#15489-H07E)
Species Reactivity
Human GPD1 / Glycerolphosphate
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Human GPD1 / Glycerolphosphate Dehydrogenase 1 (rh GPD1 / Glycerolphosphate Dehydrogenase 1; Catalog#15489-H07E; P21695; Met1-Met349). GPD1 / Glycerolphosphate Dehydrogenase 1 specific IgG was purified by Human GPD1 / Glycerolphosphate Dehydrogenase 1 affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-Glycerol 3 Phosphate Dehydrogenase Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-GPD-CALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-GPDH-CALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-HTGTIALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
Glycerol 3 Phosphate Dehydrogenase Background Information
GPD1, also known as glycerolphosphate dehydrogenase 1, is a member of the NAD-dependent glycerol-3-phosphate dehydrogenase family. GPD1 catalyzes the reversible redox conversion of dihydroxyacetone phosphate (DHAP), thus plays a critical role in carbohydrate and lipid metabolism. It also reduces nicotine adenine dinucleotide (NADH) to glycerol-3-phosphate (G3P) and NAD+. Meanwhile, GPD1 and mitochondrial glycerol-3-phosphate dehydrogenase also form a glycerol phosphate shuttle that facilitates the transfer of reducing equivalents from the cytosol to mitochondria. Mutations in GPD1 gene are a cause of transient infantile hypertriglyceridemia.
Full Name
glycerol-3-phosphate dehydrogenase 1 (soluble)
References
Ou X. et al., 2006, J Mol Biol. 357 (3): 858-69. Ou Xianjin. et al., 2011, Journal of Molecular Biology. 357 (3): 858-69. Harding Jr. et al., 1975, Biochem Journal. 146: 223-9.
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