Anti-ADPRH Magnetic Beads-IP Kit Product Components
Components | Storage |
Anti-ADPRH Magnetic Beads1,3 | 2-8℃ for 12 months |
NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
5×TBST(pH7.4) | |
1×TBST(pH7.4) | |
ddH2O | |
CD166 Positive Cell Lysate | -20℃ for 12 months |
Alkaline Elution Buffer | 2-8℃ for 12 months |
Acidity Elution Buffer | 2-8℃ for 12 months |
Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-ADPRH magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-ADPRH Magnetic Beads-IP Kit Product Description
The Anti-ADPRH magnetic Beads, conjugated with Anti-ADPRH antibody, are used for immuneprecipitation (IP) of ADPRH proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing ADPRH proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound ADPRH proteins are dissociated from the magnetic beads using an elution buffer. Anti-ADPRH Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Human ADPRH Protein (Catalog#15393-H07E)
Species Reactivity
Human ADPRH
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Human ADPRH (rh ADPRH; Catalog#15393-H07E; NP_001116.1; Met1-Leu351). ADPRH specific IgG was purified by Human ADPRH affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-ADPRH Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-ARH1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
ADPRH Background Information
Cholera toxin (CT) produced by Vibrio cholerae causes the devastating diarrhea of cholera by catalyzing the ADP-ribosylation of the alpha subunit of the intestinal Gs protein (Gsalpha), leading to characteristic water and electrolyte losses. Mammalian cells contain ADP-ribosyltransferases similar to CT and an ADP-ribosyl(arginine)protein hydrolase (ADPRH), which cleaves the ADP-ribose-(arginine)protein bond, regenerating native protein and completing an ADP-ribosylation cycle. CT-catalyzed ADP-ribosylation of cell proteins can be counteracted by ADPRH, which could function as a modifier gene in disease. Further, our study demonstrates that enzymatic cross talk exists between bacterial toxin ADP-ribosyltransferases and host ADP-ribosylation cycles. In disease, toxin-catalyzed ADP-ribosylation overwhelms this potential host defense system, resulting in persistence of ADP-ribosylation and intoxication of the cell. Mono-ADP-ribosylation is a reversible modification of proteins with NAD:arginine ADP-ribosyltransferases and ADP-ribosylarginine hydrolases (ADPRH) catalyzing the opposing arms of an ADP-ribosylation cycle. The ADPRH cDNA had been cloned from human, rat, and mouse tissues and high levels of mRNA were found in brain, spleen, and testis. Human ADP-ribosylhydrolase 1 (hARH1, ADPRH) cleaves the glycosidic bond of ADP-ribose attached to an Arg residue of a protein.
Full Name
ADP-ribosylarginine hydrolase