Anti-DCUN1D1 Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-DCUN1D1 Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-DCUN1D1 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-DCUN1D1 Magnetic Beads-IP Kit Product Description
The Anti-DCUN1D1 magnetic Beads, conjugated with Anti-DCUN1D1 antibody, are used for immuneprecipitation (IP) of DCUN1D1 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing DCUN1D1 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound DCUN1D1 proteins are dissociated from the magnetic beads using an elution buffer. Anti-DCUN1D1 Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Human DCUN1D1 / SCCRO protein (Catalog#15059-H07E)
Species Reactivity
Human DCUN1D1 / SCCRO
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Human DCUN1D1 / SCCRO (rh DCUN1D1 / SCCRO; Catalog#15059-H07E; NP_065691.2; Met1-Val259). DCUN1D1 / SCCRO specific IgG was purified by Human DCUN1D1 / SCCRO affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-DCUN1D1 Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-DCNL1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-DCUN1L1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-RP42ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-SCCROALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-SCROALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-Tes3ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
DCUN1D1 Background Information
DCUN1D1, also known as SCCRO, is part of an E3 ubiquitin ligase complex for neddylation. DCUN1D1 functions to recruit charged E2 and is involved in the release of inhibitory effects of CAND1 on cullin-RING ligase E3 complex assembly and activity. DCUN1D1 binds to the components of the neddylation pathway (Cullin-ROC1, Ubc12, and CAND1) and augments but is not required for cullin neddylation in reactions using purified recombinant proteins. DCUN1D1 also recruits Ubc12 approximately NEDD8 to the CAND1-Cul1-ROC1 complex but that this is not sufficient to dissociate or overcome the inhibitory effects of CAND1 on cullin neddylation in purified protein assays. DCUN1D1 also acts as am ncogene facilitating malignant transformation and carcinogenic progression.
Full Name
DCN1, defective in cullin neddylation 1, domain containing 1
References
Heir P. et al., 2013, Mol Cell Biol. 33 (8): 1621-31. Kurz T. et al., 2005, Nature. 435 (7046): 1257-61. Kim AY. et al., 2008, J Biol Chem. 283 (48): 33211-20.
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