Anti-CEBP gamma Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-CEBP gamma Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-CEBP gamma magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-CEBP gamma Magnetic Beads-IP Kit Product Description
The Anti-CEBP gamma magnetic Beads, conjugated with Anti-CEBP gamma antibody, are used for immuneprecipitation (IP) of CEBP gamma proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing CEBP gamma proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound CEBP gamma proteins are dissociated from the magnetic beads using an elution buffer. Anti-CEBP gamma Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Human CEBPG / CEBP gamma Protein (Catalog#14925-H07E)
Species Reactivity
Human CEBPG / CEBP gamma
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Human CEBPG / CEBP gamma (rh CEBPG / CEBP gamma; Catalog#14925-H07E; P53567; Pro39-Asn147). CEBPG / CEBP gamma specific IgG was purified by Human CEBPG / CEBP gamma affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-CEBP gamma Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-GPE1BPALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-IG/EBP-1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
CEBP gamma Background Information
CEBPG, also known as CEBP gamma, is a transcription factor which belongs to the CEBP family. Members of this family regulate viral and cellular CCAAT/enhancer element-mediated transcription. CEBP proteins contain the bZIP region, which is characterized by two motifs in the C-terminal half of the protein: a basic region involved in DNA binding and a leucine zipper motif involved in dimerization. CEBPG binds to the enhancer element PRE-I of the IL-4 gene. It Might change the DNA-binding specificity of other transcription factors and recruit them to unusual DNA sites.
Full Name
CCAAT/enhancer binding protein (C/EBP), gamma
References
Thomassin H. et al., 1992, Nucleic Acids Res. 20 (12): 3091-8. Nishizawa M. et al., 1992, FEBS Lett. 299 (1): 36-8. Williams SC. et al., 1991, Genes Dev. 5 (9): 1553-67. Melchionna R. et al., 2012, J Invest Dermatol. 132 (7): 1908-17.
新浪微博
腾讯微博
QQ空间
客服
