Anti-CARKL Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-CARKL Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-CARKL magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-CARKL Magnetic Beads-IP Kit Product Description
The Anti-CARKL magnetic Beads, conjugated with Anti-CARKL antibody, are used for immuneprecipitation (IP) of CARKL proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing CARKL proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound CARKL proteins are dissociated from the magnetic beads using an elution buffer. Anti-CARKL Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Human CARKL / SHPK protein (Catalog#14833-H20B)
Species Reactivity
Human CARKL / SHPK
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Human CARKL / SHPK (rh CARKL / SHPK; Catalog#14833-H20B; NP_037408.2; Ala2-Ser478). CARKL / SHPK specific IgG was purified by Human CARKL / SHPK affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-CARKL Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-CARKLALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-SHKALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
CARKL Background Information
CARKL, also known as SHPK, is a nonprotein kinase of glucose metabolism. CARKL has weak homology to several carbohydrate kinases, a class of proteins involved in the phosphorylation of sugars as they enter a cell, inhibiting return across the cell membrane. CARKL catalyzes an orphan reaction in the pentose phosphate pathway, refocusing cellular metabolism to a high-redox state upon physiological or artificial downregulation. CARKL-dependent metabolic reprogramming is required for proper M1- and M2-like macrophage polarization and uncover a rate-limiting requirement for appropriate glucose flux in macrophage polarization.
Full Name
sedoheptulokinase
References
Haschemi A. et al., 2012, Cell Metab. 15 (6): 813-26. Udeshi ND. et al., 2012, Mol Cell Proteomics. 11 (5): 148-59. Wamelink MM. et al., 2008, Hum Mutat. 29 (4): 532-6.
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