Anti-WARS Magnetic Beads-IP Kit Product Components
Components | Storage |
Anti-WARS Magnetic Beads1,3 | 2-8℃ for 12 months |
NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
5×TBST(pH7.4) | |
1×TBST(pH7.4) | |
ddH2O | |
CD166 Positive Cell Lysate | -20℃ for 12 months |
Alkaline Elution Buffer | 2-8℃ for 12 months |
Acidity Elution Buffer | 2-8℃ for 12 months |
Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-WARS magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-WARS Magnetic Beads-IP Kit Product Description
The Anti-WARS magnetic Beads, conjugated with Anti-WARS antibody, are used for immuneprecipitation (IP) of WARS proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing WARS proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound WARS proteins are dissociated from the magnetic beads using an elution buffer. Anti-WARS Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Human WARS / TrpRS protein (Catalog#14827-H07B)
Species Reactivity
Human WARS / TrpRS
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Human WARS / TrpRS (rh WARS / TrpRS; Catalog#14827-H07B; P23381-1; Pro2-Gln471). WARS / TrpRS specific IgG was purified by Human WARS / TrpRS affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-WARS Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-GAMMA-2ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-IFI53ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-IFP53ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
WARS Background Information
WARS, also known as TrpRS, is an aminoacyl-tRNA synthetase which belongs to the class-I aminoacyl-tRNA synthetase family. There are two forms of tryptophanyl-tRNA synthetase: a cytoplasmic form, named WARS, and a mitochondrial form, named WARS2. They catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. WARS catalyzes the aminoacylation of tRNA(trp) with tryptophan and is induced by interferon.
Full Name
tryptophanyl-tRNA synthetase
References
Buwitt U. et al., 1992, EMBO J. 11 (2): 489-96. Fleckner J. et al., 1992, Proc Natl Acad Sci. 88 (24): 11520-4. Ewalt KL. et al., 2002, Biochemistry. 41 (45): 13344-9.