Anti-CSEN Magnetic Beads-IP Kit Product Components
Components | Storage |
Anti-CSEN Magnetic Beads1,3 | 2-8℃ for 12 months |
NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
5×TBST(pH7.4) | |
1×TBST(pH7.4) | |
ddH2O | |
CD166 Positive Cell Lysate | -20℃ for 12 months |
Alkaline Elution Buffer | 2-8℃ for 12 months |
Acidity Elution Buffer | 2-8℃ for 12 months |
Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-CSEN magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-CSEN Magnetic Beads-IP Kit Product Description
The Anti-CSEN magnetic Beads, conjugated with Anti-CSEN antibody, are used for immuneprecipitation (IP) of CSEN proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing CSEN proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound CSEN proteins are dissociated from the magnetic beads using an elution buffer. Anti-CSEN Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Human CSEN / calsenilin / KCNIP3 Protein (Catalog#14683-H07E)
Species Reactivity
Human CSEN / calsenilin / KCNI
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Human CSEN / calsenilin / KCNIP3 (rh CSEN / calsenilin / KCNIP3; Catalog#14683-H07E; Q9Y2W7-1; Met1-Ile256). CSEN / calsenilin / KCNIP3 specific IgG was purified by Human CSEN / calsenilin / KCNIP3 affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-CSEN Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-CSENALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-DREAMALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-KCHIP3ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
CSEN Background Information
CSEN, also known as calsenilin and KCNIP3, is a member of the family of voltage-gated potassium (Kv) channel-interacting proteins, which belong to the recoverin branch of the EF-hand superfamily. Members of this family are integral subunit components of native Kv4 channel complexes that may regulate A-type currents, and hence neuronal excitability, in response to changes in intracellular calcium. CSEN also functions as a calcium-regulated transcriptional repressor, and interacts with presenilins. CSEN binds to the DRE element of genes including PDYN and FOS.
Full Name
Kv channel interacting protein 3, calsenilin
References
Buxbaum J.D., et al.,(1998), Calsenilin: a calcium-binding protein that interacts with the presenilins and regulates the levels of a presenilin fragment. Nat. Med. 4:1177-1181. Carrion A.M., et al., (1999), DREAM is a Ca2+-regulated transcriptional repressor.Nature 398:80-84. An W.F., et al.,(2000), Modulation of A-type potassium channels by a family of calcium sensors.Nature 403:553-556.