Anti-ETHE1 Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-ETHE1 Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-ETHE1 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-ETHE1 Magnetic Beads-IP Kit Product Description
The Anti-ETHE1 magnetic Beads, conjugated with Anti-ETHE1 antibody, are used for immuneprecipitation (IP) of ETHE1 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing ETHE1 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound ETHE1 proteins are dissociated from the magnetic beads using an elution buffer. Anti-ETHE1 Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Human ETHE1 / HSCO protein (Catalog#14681-H07E)
Species Reactivity
Human ETHE1 / HSCO
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Human ETHE1 / HSCO (rh ETHE1 / HSCO; Catalog#14681-H07E; O95571; Leu13-Ala254). ETHE1 / HSCO specific IgG was purified by Human ETHE1 / HSCO affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-ETHE1 Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-HSCOALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-YF13H12ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
ETHE1 Background Information
ETHE1, also known as HSCO, is a sulfur dioxygenase that localizes within the mitochondrial matrix. ETHE1 probably plays an important role in metabolic homeostasis in mitochondria. It may also function as a nuclear-cytoplasmic shuttling protein that binds transcription factor RELA/NFKB3 in the nucleus and exports it to the cytoplasm. ETHE1 can suppresses p53-induced apoptosis by preventing nuclear localization of RELA. Mutations in ETHE1 gene result in ethylmalonic encephalopathy. Ethylmalonic encephalopathy is an autosomal recessive, invariably fatal disorder characterized by early-onset encephalopathy, microangiopathy, chronic diarrhea, defective cytochrome c oxidase (COX) in muscle and brain, high concentrations of C4 and C5 acylcarnitines in blood and high excretion of ethylmalonic acid in urine.
Full Name
ethylmalonic encephalopathy 1
References
Higashitsuji. et al., 2002, Cancer Cell. 2 (4): 335-46. McCoy JG. et al., 2007, Acta Crystallogr D Biol Crystallogr. 62 (9): 964-70. Mehrle A. et al., 2006, Nucleic Acids Res. 34: D415-8.
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