Anti-TCTP Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-TCTP Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-TCTP magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-TCTP Magnetic Beads-IP Kit Product Description
The Anti-TCTP magnetic Beads, conjugated with Anti-TCTP antibody, are used for immuneprecipitation (IP) of TCTP proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing TCTP proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound TCTP proteins are dissociated from the magnetic beads using an elution buffer. Anti-TCTP Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Human TCTP / TPT1 protein (Catalog#14662-H07E)
Species Reactivity
Human TCTP / TPT1
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Human TCTP / TPT1 (rh TCTP / TPT1; Catalog#14662-H07E; TCTP_HUMAN; Met1-Cys172). TCTP / TPT1 specific IgG was purified by Human TCTP / TPT1 affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-TCTP Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-HRFALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-p02ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-p23ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-TCTPALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
TCTP Background Information
Tumor protein, also known as TPT1, is a highly conserved protein among many eukaryotic organisms. Tumor protein is involved in a variety of cellular activities, including microtubule stabilization, calcium-binding activities, and apoptosis. The Mammalian translationally controlled tumour protein (TPT1) (or P23) is a protein which has been found to be preferentially synthesised in cells during the early growth phase of some types of tumour, but which is also expressed in normal cells. It was first identified as a histamine-releasing factor, acting in IgE +-dependent allergic reactions. In addition, TPT1 has been shown to bind to tubulin in the cytoskeleton, has a high affinity for calcium, is the binding target for the antimalarial compound artemisinin, and is induced in vitamin D-dependent apoptosis. TPT1 production is thought to be controlled at the translational as well as the transcriptional level.
Full Name
tumor protein, translationally-controlled 1
References
Thaw P. et al., 2001, Nat Struct Biol. 8 (8): 701-4. Thiele H. et al., 2000, Eur J Biochem. 267 (17): 5473-81. Chitpatima ST. et al., 1988, Nucleic Acids Res. 16 (5): 2350.
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