Anti-PMM2 Magnetic Beads-IP Kit Product Components
Components | Storage |
Anti-PMM2 Magnetic Beads1,3 | 2-8℃ for 12 months |
NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
5×TBST(pH7.4) | |
1×TBST(pH7.4) | |
ddH2O | |
CD166 Positive Cell Lysate | -20℃ for 12 months |
Alkaline Elution Buffer | 2-8℃ for 12 months |
Acidity Elution Buffer | 2-8℃ for 12 months |
Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-PMM2 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-PMM2 Magnetic Beads-IP Kit Product Description
The Anti-PMM2 magnetic Beads, conjugated with Anti-PMM2 antibody, are used for immuneprecipitation (IP) of PMM2 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing PMM2 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound PMM2 proteins are dissociated from the magnetic beads using an elution buffer. Anti-PMM2 Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Human phosphomannomutase 2 / PMM2 / CDG1 protein (Catalog#14648-H07E)
Species Reactivity
Human phosphomannomutase 2 / P
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Human phosphomannomutase 2 / PMM2 / CDG1 (rh phosphomannomutase 2 / PMM2 / CDG1; Catalog#14648-H07E; O15305; Met1-Ser246). phosphomannomutase 2 / PMM2 / CDG1 specific IgG was purified by Human phosphomannomutase 2 / PMM2 / CDG1 affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-PMM2 Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-CDG1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-CDG1aALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-CDGSALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-PMIALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-PMI1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-PMM2ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
PMM2 Background Information
Phosphomannomutase 2, also known as PMM2 and CDG1, belongs to the eukaryotic PMM family. Phosphomannomutase 2 catalyzes the isomerization of mannose 6-phosphate to mannose 1-phosphate. Mannose 1-phosphate is a precursor to GDP-mannose necessary for the synthesis of dolichol-P-oligosaccharides. GDP-mannose can transfer its small sugar molecule called mannose to the growing oligosaccharide chain. Once the correct number of small sugar molecules are linked together to form the oligosaccharide, it can be attached to a protein. Phosphomannomutase 2 is also required for a number of critical mannosyl transfer reactions. Mutations in PMM2 gene have been shown to cause defects in the protein glycosylation pathway manifest as carbohydrate-deficient glycoprotein syndrome type I.
Full Name
phosphomannomutase 2
References
Jaeken J. et al., 2002, Annual review of genomics and human genetics. 2: 129-51. Matthijs G. et al., 2000, Mol Genet Metab. 68 (2): 220-6. Matthijs G. et al., 1997, Nat Genet. 16 (1): 88-92.